Research Programmes
Do you know…
- … the scientists in CEITEC will be researching what happens in the brain when a human has realized that they have made a mistake?
- … there will be nearly 800 scientists in CEITEC?
- … in CEITEC we will be researching how the brain works and why some people are motivated and others are not?
- … thanks to CEITEC most of the diagnostic methods will be cheaper, faster and more comfortable for the patients?
- … 63 research teams will be created in CEITEC?
- … there will be 7 research programmes in CEITEC?
- … there will be more than 1,000 modern instruments in CEITEC?
- … more than 31,000 m2 of new infrastructure will be built in CEITEC?
- … more than 1,500 students will use the CEITEC infrastructure per year?
- … there will be 10 shared laboratories created in CEITEC?
- … CEITEC has 6 partners?
- … work will be carried out on self-cleaning surfaces in CEITEC?
- … CEITEC will co-operate closely with the industrial sector?
- … there will also be international scientists working in CEITEC?
- … CEITEC will support the international mobility of scientists?
- … the scientists in CEITEC are developing a subdermal chip which will analyse some life functions and will inform doctors from a distance?
- … the scientists in CEITEC are working on the development of a device which will enable physiotherapy from a distance?
- … the scientists at CEITEC are working on the development of biosensors?
- … CEITEC will be created in the south-Moravian city of Brno?
Experimental Biophotonics
Prof. RNDr. Radim Chmelík, Ph.D.
Research Group Leader
THEMATIC RESEARCH FOCUS
RESEARCH AREAS
- Imaging process in advanced, especially holographic and confocal light microscopy: theory, simulation and experimental verifi cation
- Coherence effects, optical and coherence vortices in holographic imaging
- Coherence controlled optical systems for holographic and multimodal (correlative) microscopy
- Advanced image-processing methods: digital holographic reconstruction and numerical focusing with low coherence, phase unwrapping in presence of coherence vortices dynamical phase diff erences, pattern & process recognition
- Biophysics of quantitative evaluation of live cell behaviour developed by coherence-controlled holographic microscopy (CCHM)
- Subtoxic impact/toxicity of nanomaterials assessment by measurement of intracellular dry mass translocation by CCHM
- Observation and validation of nanostructures properties by advanced light microscopy
- In vitro qualification of cancer cell malignancy by CCHM classifi cation of cell behaviour patterns
MAIN OBJECTIVES
Research and development of analytical and measurement methods
Experimental Biophotonics is focussed on advanced light microscopy techniques and methodologies applied to study of nano- and microstructures characteristics and interactions with living cells including assessment of possible cytotoxicity and cancer cell diagnostics.
CONTENT OF RESEARCH
Research and development of instruments and methods for the investigation of nanomaterials and nanostructures
Optical methods
The development of the methodology for photoluminescence and refl ection optical microscopy and spectroscopy of nanosystems (including near-fi eld methods).
The development and application of confocal and holographic microscopy and optical spectroscopic methods (ellipsometry and spectrophotometry) in a wide spectral range from vacuum ultraviolet (VUV) to infrared (IR) range.
The development of structural and dispersion models and software for the analysis of these measurements.
Experimental biophotonics work is focussed to the segment of development and application of confocal and holographic microscopy focussed on nano- and microstructures characterisation and their interactions with living cells including assessment of possible cytotoxicity, and on applications to in-vitro cancer cell biology.
KEY RESEARCH EQUIPMENT
PLANNED RESEARCH INFRASTRUCTURE
Core Facilities
The research group will be one of the principal users of the equipment available within CEITEC Nanolithography and Nanofabrication and Nanocharacterisation Core Facilities.
Technology Units
Experimental biophotonics
CURRENT RESEARCH INFRASTRUCTURE
Complete equipment for research and development in the field of advanced light microscopy: design and simulation software, optical tables, opto-mechanical components, optical kits, halogen, xenon and laser sources, digital cameras.
First generation of CCHM: reflected-light CCHM, transmitted-light CCHM adapted for live-cell time-lapse observations.
Basic facilities for cell-culture (GMO approved) and sample preparation: biohazard box, thermal box, inverted phase-contrast microscope.
Microscopes: Nikon Eclipse L150 with reflected light: BF, DF, DIC, fluorescence microscopy, Nikon Alphaphot 2, tandem-scanning reflected-light confocal microscope Confocal 2002.
MAIN PROJECTS
- Image reconstruction of three-dimensional objects by the methods of the holographic confocal microscopy (GA202/04/1410), Czech Science Foundation, 2004-2006, R. Chmelik, Brno University of Technology.
- The signifi cance of fast intracellular motile reactions in living cells (GA304/99/0368), Czech Science Foundation, 1999-2001, R. Chmelik, Brno University of Technology.
- Validation of digital holographic microscope in investigations of living cell dynamics & completion of new multifunctional instrument (GA202/08/0590), Czech Science Foundation, 2008-2010, R. Chmelik, Brno University of Technology.
- Inorganic nanomaterials and nanostructures: fabrication, characterization, properties (MSM0021630508), Ministry of Education, Youth and Sports, 2005-2011, J. Cihlař, Brno University of Technology.
- Engineering of the porcine genome for xenotransplantation studies in primates: a step towards clinical application (Xenome; 037377), FP6, EU, 2006-2012, Azienda Ospedaliera di Padova (coordinator).
SELECTED PUBLICATIONS
- BOUCHAL, P., KAPITAN, J., CHMELIK, R., BOUCHAL, Z. Point spread function and two-point resolution in Fresnel incoherent correlation holography. Optics Express. 2011, 19(16), p. 15603-15620.
- TOLDE, O., ROSEL, D., VESELY, P., FOLK, P., BRABEK, J. The structure of invadopodia in a complex 3D environment. Eur J Cell Biol. 2010, 89(9), p. 674-680.
- KOLMAN, P., CHMELIK, R. Coherence-controlled holographic microscope. Optics Express. 2010, 18(21), p. 21990–22003.
- JANECKOVA, H., VESELY, P., CHMELIK, R. Proving Tumour Cells by Acute Nutritional/Energy Deprivation as a Survival Threat: A Task for Microscopy. Anticancer Research. 2009, 29(6), p. 2339–2345.
- CHMELIK, R. Three-dimensional scalar imaging in high-aperture low-coherence interference and holographic microscopes. Journal of Modern Optics. 2006, 53(18), p. 2673–2689.
EUROPEAN UNION
