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… the scientists in CEITEC are developing a subdermal chip which will analyse some life functions and will inform doctors from a distance?
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Bioanalytical Instrumentation

Ing. František Foret, CSc.
Research Group Leader

THEMATIC RESEARCH FOCUS

RESEARCH AREAS

Capillary separations
Mass spectrometry coupling
Miniaturisation
Single cell analysis

MAIN OBJECTIVES

Development of novel techniques for the separation and analysis of nucleic acids, proteins, small bio active molecules & drugs and their complexes based on electrophoretic and microfluidic systems, electrochemical and optical methods and nanotechnologies.

CONTENT OF RESEARCH

Exploring new ways for achieving enhancements in resolution, sensitivity and selectivity of analyses. Utilisation of theoretical and instrumental approaches for development and applications of microfluidics, nanotechnologies and novel chemistries. Research of novel techniques for the separation and analysis of nucleic acids, proteins, small bioactive molecules & drugs and their complexes using electrophoretic and microfluidic systems coupled with electrochemical, optical and mass spectrometric methods.

Microseparation systems for the analysis of biomolecules will be developed utilising combination of microfluidic chips and structured nanoparticles including quantum dots, magnetically activated tags; enzymatic and nanoparticle modified monolithic supports.
New detection principles, based on surface signal enhancement (e.g. surface plasmon, Raman, fluorescence, electrochemistry) will be combined with the newly developed separations. These technologies and instrumentation will be developed for joint applications with other investigators of the CEITEC centre.
Technologies for microanalyses of proteins and their glycosylation will be focused on the combination of electrochemistry, microfludic biosensors and mass spectrometry for analysis of limited sample quantities. The ultimate goal of single-cell analysis will be aimed at potential application in cancer detection.

KEY RESEARCH EQUIPMENT

PLANNED RESEARCH INFRASTRUCTURE

Technology Units

Bioanalytics

CURRENT RESEARCH INFRASTRUCTURE

The current research infrastructure includes instrumentation for mass spectrometry and the separation of biomolecules.

MAIN PROJECTS

Nanotechnology in functional diagnostics of apoptotic and tumor cells (GA203/08/1680), Czech Science Foundation, 2008-2011, K. Kleparnik, Institute of Analytical Chemistry AS CR, D. Valik, Masaryk Memorial Cancer Institute, I. Mišek, Institute of Animal Physiology and Genetics.
Single-cell analysis by mass spectrometry (GAP206/11/2377), Czech Science Foundation, 2011-2013, K. Kleparnik, Institute of Analytical Chemistry AS CR.
New methods of analysis of proteins and their glycosylation in cancer – combination of electrochemistry, microfludic biosensors and mass spectrometry (P301/11/2055), Czech Science Foundation, 2011-2014, F. Foret, Institute of Analytical Chemistry AS CR, E. Paleček, Institute of Biophysics AS CR.
Integrated bioanalytical technologies for microanalyses and diagnostics with laser induced fluorescence and mass spectrometry coupling (LC06023), Ministry of Education, Youth and Sports, 2006-2011, Z. Glatz, Masaryk University, L. Fajkusova, University Hospital Brno, F. Foret, Institute of Analytical Chemistry AS CR.
Nanoparticle modified monolithic supports for bioanalysis (2SGA2721), South Moravian Programme for Distinguished Researchers (SoMoPro), 2011-2013, F. Foret, Institute of Analytical Chemistry AS CR.

SELECTED PUBLICATIONS

LISKOVA, M., VORACOVA, I., KLEPARNIK, K., HEZINOVA, V., PRIKRYL, J., FORET, F. Conjugation reactions in the preparations of quantum dot-based immunoluminescent probes for analysis of proteins by capillary electrophoresis. Anal. Bioanal. Chem. 2011, 400, p. 369-379.
SMEJKAL, P., SZEKRENYES, A., RYVOLOVA, M., FORET, F., GUTTMAN, A., BEK, F., MACKA, M. Chip-based CE for rapid separation of 8-aminopyrene-1,3,6-trisulfonic acid (APTS) derivatized glycans. Electrophoresis. 2010, 31, p. 3783–3786.
JUSKOVA, P., OSTATNA, V., PALECEK, E., FORET, F. Fabrication and Characterization of Solid Mercury Amalgam Electrodes for Protein Analysis. Anal. Chem. 2010, 82, p. 2690-2695.
TOMAS, R., KOVAL, M., FORET, F. Coupling of hydrodynamically closed large bore capillary isotachophoresis with electrospray mass spectrometry. J.Chromatogr. A. 2010, 1217, p. 4144–4149.
RYVOLOVA, M., PREISLER, J., FORET, F., HAUSER, P., C., KRASENSKY, P., PAULL, B., MACKA, M. Combined Contactless Conductometric, Photometric, and Fluorimetric Single Point Detector for Capillary Separation Methods. Anal. Chem. 2010, 82, p. 129–135.

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