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Core Facility – Proteomics

Doc. RNDr. Zbyněk Zdráhal, Dr.
Research Group Leader

THEMATIC RESEARCH FOCUS

RESEARCH AREAS

Separation of complex protein mixtures
Characterisation of proteins and their modifications by mass spectrometry
Mass spectrometric techniques for quantification of protein mixtures
MS-based proteomics in general

MAIN OBJECTIVES

To provide access to state-of-the-art technologies based on shared resources and highly trained staff in MSbased proteomics for research groups within CEITEC and outside the project.
The development of novel techniques for the separation and analysis of proteins using mass spectrometry.

CONTENT OF RESEARCH

Our research interests are focused on the field of MS-based proteomics including isolation of protein symplex from various matrices, fractionation and separation of complex protein mixtures by chromatographic and electrophoretic techniques, and namely on application of mass spectrometric techniques for qualitative and quantitative characterisation of proteins and their modifications.

At present, we participate on several research projects with diverse topics in area of molecular biology, biomedicine and agriculture. Our roles within these projects represent: development and aplication of MALDI-MS profiling for taxonomic studies of selected bacterial genera and funghi, characterisation of differentially expressed proteins (e.g. plant proteins after hormonal treatment), characterisation of protein complexes, characterisation of histone acetylations and assessment of the influence of selected histone deacetylase inhibitors on histone acetylation state, characterisation of phosphorylation state of selected proteins, characterisation of group of patatin proteins in different potato cultivars or searching for potentional disease biomarkers in blood plasma.

KEY RESEARCH EQUIPMENT

PLANNED RESEARCH INFRASTRUCTURE

Proteomics Core Facility

CF Proteomics – Mass spectrometry
CF Proteomics – Protein separation
CF Proteomics – Sample storage and preparation
CF Proteomics – Protein separation

CURRENT RESEARCH INFRASTRUCTURE

The current infrastructure includes protein isolation, protein separation and mass spectrometry.

MAIN PROJECTS

Functional characterization of pollen unique storage ribonucleoprotein particles (GAP501/11/1462), Czech Science Foundation, 2011-2014, D. Honys, Institute of Experimental Botany AS CR, Z. Zdráhal, Masaryk University.
Methods of eradication of production diseases of cattle – bovine viral diarrhoea – mucosal disease and paratuberculosis (QI101A094), Ministry of Agriculture, 2010-2013, M. Faldyna, Institute of Veterinary Research, Z. Zdráhal, Masaryk University, V. Celer, University of Veterinary and Pharmaceutical Sciences Brno, J. Kucera, Farmers Union of Czech Pied Cattle.
Development of diagnostics and treatment of serious heart and vascular diseases using genomic and proteomic approaches (2B08060), Ministry of Education, Youth and Sports, 2008-2011, L. Fajkusová, University Hospital Brno, T. Freiberger, Cardiovascular and Transplant Surgery Center, Z. Zdráhal, Masaryk University.
Molecular basis of cell and tissue regulations (MSM0021622415), Ministry of Education, Youth and Sports, 2005-2011, J. Fajkus, Masaryk University.

SELECTED PUBLICATIONS

SEDO, O., SEDLACEK, I., ZDRAHAL, Z. Sample preparation methods for MALDI-MS profiling of bacteria. Mass Spectrom. Rev. 2011, 30(3), p. 417-434.
STOPKOVA, P., ZDRAHAL, Z., RYBA, S., SEDO, O., SANDERA, M., STOPKA, P. Novel OBP genes similar to hamster Aphrodisin in the bank vole, Myodes glareolus. BMC Genomics. 2010, 11(45), 10 pp.
LOCHMANOVA, G., ZDRAHAL, Z., KONECNA, K., KOUKALOVA, S., MALBECK, J., SOUCEK, P., VALKOVA, M., KIRAN, N., S., BRZOBOHATY, B. Cytokinin induced photomorphogenesis in dark-grown Arabidopsis: A proteome analysis. J. Exp. Bot. 2008, 59(13), p. 3705-3719.
EYER, L., PANTUCEK, R., ZDRAHAL, Z., KONECNA, H., KASPAREK, P., RUZICKOVA, V., HERNYCHOVA, L., PREISLER, J., DOSKAR, J. Structural protein analysis of the polyvalent staphylococcal bacteriophage 812. Proteomics. 2007, 7(1), p. 64-72.
BENES, P., MACECKOVA, V., ZDRAHAL, Z., KONECNA, H., ZAHRADNICKOVA, E., SMARDA, J. Role of vimentin in regulation of monocyte/macrophages. Differentiation. 2006, 74(6), p. 265-276.

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