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Food Safety

Prof. MVDr. Iva Steinhauserová, CSc.
Research Group Leader

THEMATIC RESEARCH FOCUS

RESEARCH AREAS

Cultivation and identification of foodborne pathogens
Molecular biology characterisation of foodborne pathogens
Monitoring of zoonotic pathogens in food chain
Testing of resistance and survival of selected food-borne pathogens under different processing conditions

MAIN OBJECTIVES

To obtain novel information on the causes, mechanisms and spreading of infectious diseases in domestic animals.
Identification of mechanisms and prevention of the circulation of zoonotic pathogens and commensal antimicrobial-resistant bacteria in the food chain.

CONTENT OF RESEARCH

Analysis and the prevention of the circulation of zoonotic pathogens in the food chain

Pathogenic and potentially pathogenic bacteria are nowadays of great interest from the food safety point of view. The contamination of food matrices will be studied by culture independent methods based on PCR and real time PCR in order to determine the impact of food processing on pathogens’ presence and survival. The aims are monitoring, typing / subtyping, quantitation of selected microorganisms, and identification of genes responsible for the resistance and survival of selected food-borne pathogens under stress conditions used in the food industry.

Selected food-borne pathogens (e.g. Campylobacter spp., Listeria sp., Salmonella spp.) will be monitored from farm to consumers. Isolated strains from various types of farms (conventional and organic), processing plants and from patients suffering from food-borne diseases will be characterised by phenotyping and genotyping methods. The resistance and survival of selected food-borne pathogens under different processing conditions will be studied directly in food samples.

KEY RESEARCH EQUIPMENT

PLANNED RESEARCH INFRASTRUCTURE

Technology Units

Characterisation of pathogenic isolates – determination of relations of isolated clones, assessment of resistance
Detection of pathogens using culture and molecular techniques

CURRENT RESEARCH INFRASTRUCTURE

The Department of Meat Hygiene and Technology has laboratories for the isolation, growth and storage of pathogens and other infective materials. Specific laboratory units enable them to detect and characterise pathogenic microorganisms using molecular biology methods. The department has a technological laboratory with the possibility of performing technological experiments with pathogenic microorganisms and simulating real conditions.

MAIN PROJECTS

Innovative Methods of Manufacturing, Cutting and Packaging of Meat Products, 2010, L. Steinhauser, Steinhauser, Ltd., I. Steinhauserová, University of Veterinary and Pharmaceutical Sciences Brno, Krásno Meat Industry, Inc.
POULTRYFLORGUT – Control of the intestinal flora in poultry for ensuring the products safety for human consumers (7076), FP6-FOOD, EU, 2005-2008, I. Steinhauserová, University of Veterinary and Pharmaceutical Sciences Brno.
Veterinary aspects of food safety and quality (MSM6215712402), Ministry of Education, Youth and Sports, 2005-2011, V. Večerek, University of Veterinary and Pharmaceutical Sciences Brno.

SELECTED PUBLICATIONS

NEBOLA, M., BORILOVA, G., KASALOVA, J. PCR-RFLP Analysis of DNA for Differentiation of Fish Species in Seafood Samples. Bulletin of the Veterinary Institute in Pulawy. 2010, 54, p. 49-53.
FAJT, Z., MODRA, H., BANOCH, T., BLAHOVA, J., SALAKOVA, A., STEINHAUSEROVA, I., SVOBODA, M. Effect of high dietary levels of Se-enriched yeast on muscle selenium content and meat quality traits – a model study in rats. Neuroendocrinology Letters. 2010, 31(2), p. 114-119.
DE CESARE, A., BORILOVA, G., SVOBODOVA, I., BONDIOLI, V., MANFREDA, G. Clostridium perfringens occurrence and ribotypes in healthy broilers reared in different European countries. Poultry Science. 2009, 88(9), p. 1850-1857.
BORILOVA, G., NEBOLA, M., STEINHAUSEROVA, I. Occurrence and antibiotic resistance of Campylobacter spp. isolated from pheasants (Phasianus colchicus spp. torquatus). Archiv für Lebensmittelhygiene. 2007, 58(5), p. 116-120.
NEBOLA, M., STEINHAUSEROVA, I. PFGE and PCR/RFLP typing of Campylobacter jejuni strains from poultry. British Poultry Science. 2006, 47(4), p. 456-461.

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