Bioanalytical Instrumentation - František Foret
CEITEC MU CEITEC MU
Bioanalytical Instrumentation - František Foret
Bioanalytical Instrumentation

PhD Topics

1. Development of capillary electrophoresis methods for non-invasive diagnostics of gastroesophageal reflux disease

Supervisor: assoc. prof. Petr Kubáň

Consultant: Ing. František Foret

Annotation: Gastroesophageal reflux is the most common upper gastrointestinal tract disorder that is highly prevalent worldwide with a prevalence of 10%-20% in the western world. Currently the diagnostic methods for gastroesophageal reflux disease (GERD) are all invasive and for instance the 24-hour intraluminal impedance – pH measurement is not well tolerated by majority of the diagnosed patients. Non-invasive samples of biological fluids, such as saliva and exhaled breath condensate are promising samples that could be used for detection and quantitation of selected biomarkers that will help in non-invasive diagnostics of GERD.

In this Ph.D. topic these non-invasive samples will be analyzed by selected capillary electrophoretic methods with the aim to identify relevant compounds that are able to distinguish the samples of GERD patients and healthy controls. The work will include the optimization of the sampling of saliva and exhaled breath condensate and subsequent analysis of selected biomarkers in these samples by capillary electrophoresis.

RG: František Foret Research Group

Keywords: gastroesophageal reflux, GERD, non-invasive samples, capillary electrophoresis, microcolumn separation methods, targeting biomarkers, mass spectrometry

 

2. Development of portable capillary electrophoresis instrumentation for medical diagnostic purposes

Supervisor: assoc. prof. Petr Kubáň

Consultant: Ing. František Foret

Annotation: Clinical diagnostics of selected diseases requires analysis of multicomponent mixtures of analytes that is often difficult to accomplish by conventional methods approved for clinical practice. The use of more sophisticated techniques such as HPLC is however restricted to biochemical laboratories, because the instruments are not portable and require significant expertize. Capillary electrophoresis is a separation technique that can be easily made portable, as it does not use instrument parts that need the mains power. Additionally, for capillary electrophoresis, numerous open source literature resources are available, making it possible to construct a fully open source, microcontroller controlled CE instrument that can be used for fully automated analysis and even data evaluation of the results. In this Ph.D. topic a microcomputer operated, portable capillary electrophoresis instrument will be developed with various detection schemes (UV, C4D, LIF) with a major emphasis on simple operation, automation and possibly automated data analysis. The developed instrument will be used in various tasks related to clinical diagnosis in cooperation with the faculty hospital in Brno.

RG: František Foret Research Group

Keywords: non-invasive samples, capillary electrophoresis, microcolumn separation methods, targeting biomarkers, mass spektrometry