We use NMR and hybrid structural methods, as well as biophysical techniques, to study protein-DNA complexes involved in DNA repair and transcription. Both topics involve proteins that are promising targets for anti-cancer drugs. Therefore the group has a strong interest in structure-based drug design.
Protein-DNA transactions are essential for genome maintenance. We use structural, biochemical, and computational tools to study the function of DNA-repair factors. We employ a combination of solution techniques (NMR, SAXS-SANS, FRET) to structurally characterize protein-DNA complexes and understand dynamic rearrangements induced upon DNA binding. Structural findings are complemented in a broader functional context by genetic, molecular or cell biology, and single-molecule assays (L. Krejci, Masaryk University).
DNA repair as a therapeutic target has received considerable attention owing to the promise of drugs that target DNA-repair enzymes and potentiate conventional cytotoxic therapy through mechanism-based approaches. In this direction, we study the binding of small molecules to protein targets of interest, in order to modulate their function for potential therapeutic benefit. Promising inhibitors are identified based on in silico design (Prof. E. Mikros, University of Athens; Prof. J. Tuszynski, University of Alberta) or high-throughput screening (P. Bartunek, IMG Prague).
list / cards
Name and position
|Konstantinos Tripsianes, Ph.D.
Research Group Leader
|+420 54949 6607|
|+420 54949 7834|
|+420 54949 7834|
Supervisor: Konstantinos Tripsianes, Ph.D.
Consultants: doc. Mgr. Lumír Krejčí, Ph.D.
RecQ helicases are ubiquitous enzymes involved in the maintenance of genome stability, acting in DNA repair, replication, and recombination. Germ line mutations in genes coding for three of five human RecQ enzymes are associated with autosomal recessive disorders, characterized by increased genome instability, premature aging, and cancer predisposition. In this project, we aim to characterize regulatory domains of human RecQ helicases to understand the specific role of RecQ enzymes within the cell. We will undertake structural methods, such as NMR Spectroscopy, SAXS, and X-ray Crystallography, in combination with a wide range of biophysical and biochemical techniques to clarify the structural determinants for DNA binding and protein-protein interactions. The structural findings will be evaluated in a cell biology setting to decipher the molecular mechanism of action for this class of enzymes.
22. září 2017 12:44
WHO? Dr. Zoltan Racz WHAT ABOUT? Paper-based Electronics – Materials, Fabrication and Applications WHEN? Tuesday: September, 26 starts 13:…
22. září 2017 10:34
WHEN: September 29, 2017 from 14:00 WHERE: Room 145, building A35, University Campus Bohunice SPEAKER: Prof. Renée Schroeder, …