CF: Core Facility Biomolecular Interaction and Crystallization
CEITEC MU CEITEC MU
Core Facility Biomolecular Interaction and Crystallization

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Head of Core Facility

Prof. Michaela Wimmerová, Ph.D. Prof. Michaela Wimmerová, Ph.D. Research Group Leader, Head of Core Facility
Phone: +420 54949 3805, +420 54949 8166
Email:
Office:

Official Email: bic@ceitec.muni.cz


Main Activity

The core facility provides services leading to structure characterization of biomolecules and to study (bio)molecular interactions in a real time using mainly biosensor and calorimetry-based methods. It is equipped with the instrumentation to set up crystallization conditions of biomolecules and their complexes, basic characterization of physical properties of the molecules (analytical ultracentrifugation, dynamic light scattering, CD spectroscopy, differential scanning calorimetry), and to study thermodynamics and/or kinetics of interactions (isothermal titration calorimetry, surface plasmon resonance, CD spectroscopy, analytical ultracentrifugation).

Unique Features

  • Set-up of crystallization screens using commercial-available kits
  • Automatic inspection of crystals  - remote access to results for users
  • UV imaging to distinguish a protein from a salt
  • Set-up of optimization screens
  • Estimation of the precipitation diagram
  • Production of crystals for structural determination
  • Quality improvement of crystals for structural determination
  • Isothermal titration microcalorimetry
  • Differential scanning calorimetry
  • Surface plasmon resonance
  • Particle size analysis
  • Analytical ultracentrifugation
  • Circular dichroism spectroscopy, fluorescence measurement

Key Equipments 

The Core Facility is part of Czech national centre of European Research Infrastructure Consortium INSTRUCT ERIC under the Czech Infrastructure for Integrative Structural Biology (CIISB) funded by the Ministry of Education, Youth and Sports of the Czech Republic, and a member of an open pan-European network Association of Resources for Biophysical Research in Europe (ARBRE).