Laboratory Technician for the Research Group “RNA-based Regulation of Gene Expression”

Research Position | Published on 24.09.2018 | CEITEC MU

Laboratory Technician for the Research Group “RNA-based Regulation of Gene Expression”

Department

Peter Lukavsky Research Group – Central European Institute of Technology

 

Research Group Leader: Peter Josef Lukavsky, Ph.D.

 

Deadline

31 December 2018

Start date

Negotiable

 

A position for a laboratory technician is available in the research group “RNA-based regulation of Gene Expression” which is part of Structural Biology program at CEITEC, Masaryk University (MU). We study disease-related biological processes by investigating
protein structures and their interactions with other proteins, RNA or small molecule inhibitors using biomolecular NMR and related biophysical methods.

 

Job Description:

- Expression and purification of proteins

- Transcription and purification of RNAs

- Preparation of various media and buffers

- PCR and cloning of RNA and protein constructs

- Preparation of isotope-labelled nucleotides

- Binding studies using ITC, fluorescence anisotropy, EMSA,
and other biophysical techniques including data analysis

- Performance of NMR experiments to study small molecule interactions with proteins and RNA

- Implementing new techniques and projects

- Help incoming co-workers into a smooth start in the lab

 

Requirements:

- At least Master degree in molecular biology, biochemistry, biophysics, chemistry or other related fields of life-sciences

- Good knowledge of English (oral and written)

- Excellent interpersonal and communication skills

- High motivation, flexibility and self-driven interest to learn new things

- Reliability, team player, meticulousness

- Knowledge of "laboratory chemistry" - preparation of solutions with defined concentrations, adjusting pH, preparation of media for bacterial cultures etc.

- Computer literacy, MS word, Excel.

- Excellent experience with molecular biology, protein and RNA purifcation, biochemistry and NMR spectroscopy is a big plus

 

We offer:

- Interesting work in a top scientific team
- Stable base and excellent instrumental equipment of CEITEC MU
- employment benefits
- Salary based on qualification and experience; part time appointment possible

Location

Brno is an attractive, lively place to live and work with many cultural and recreational amenities.
Please send CV and a short motivational letter including: a) description of previous experiences b) technical skills and c) at least two contact references to Peter Lukavsky
(peter.lukavsky@ceitec.muni.cz). Candidates considered for the second round of interview will be informed via email.

Start date: Negotiable
Opened Until: 31.12.2018

I am interested
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