Prof. Vojtěch Adam, Ph.D.

Senior Researcher

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Phone: +420 54513 3350
Research group: Smart Nanodevices - Jaromír Hubálek
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2019

2018

2017

2016

2015

2014

2013

2012

2011

2010

2009

  • Nano-Electro-Bio-Tools for Biochemical and Molecularly-Biological Studies of Eukaryotic Cells (NanoBioTECell) (GAP102/11/1068), Czech Science Foundation - Standard Grants, 2011 - 2015
  • Study of contribution of different DNA-damaging mechanisms to toxicity of cytostatics to human chemosensitive and chemoresistant neuroblastomas (GAP301/10/0356), Czech Science Foundation - Standard Grants, 2010 - 2014
  • International Cooperation in the Field of Nanotechnologies with In Vivo Imaging Techniques (CZ.1.07/2.3.00/20.0148), MEYS - OP Education for Competiteveness, 2012 - 2014
  • Support of the development of high-quality teams in R&D in the field of material science (CZ.1.07/2.3.00/20.0029), MEYS - OP Education for Competiteveness, 2011 - 2014
  • Partner Network for Bionanotechnological and Metallomic Research (CZ.1.07/2.4.00/31.0023), MEYS - OP Education for Competiteveness, 2012 - 2014
  • New design and exploiting nanobiosensors and nanosensors to target medicine (KAN208130801), Academy of Sciences of the Czech Republic - Nanotechnologies for Society, 2008 - 2012

Development and application of eucaryotic cell-based microcolumn separation techniques

The thesis will focus on development of new generation of electromigration capillary separation techniques by designing, preparation and testing of novel smart interactive phases for capillary electrophoresis or capillary electrochromatography. The designed phases will be based on living cells able to selectively transform target analyte from the complex sample to a detectable product. Manufacturing phase will be based on genetic modification technology enabling not only tailor the cell receptors towards the target analyte (to be extracted from the sample and internalized into the cell) but also modify the cellular pathway for a transformation of the analyte into the product and its release back into the capillary flow.

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Synthesis and modification of magnetic particles for isolation and analysis of microbial pathogens

This work is aimed at inorganic synthesis of magnetic nanoparticles, its surface modification, characterization and testing in the area of an isolation of target molecules for subsequent chemical analysis. Produced particles will be chemically modified for selective isolation of nucleic acid from bacteria. The whole procedure of the isolation will be firstly tested using common laboratory approach and subsequently will be integrated in fluidic device. This device will be than tested for processing of samples of pathogenic bacterial strains.

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CRISPR/Cas9 system to study of secondary metabolism of unicellular algae

The aim of this thesis is to investigate of secondary metabolism of unicellular algae using genome editing based on Crispr/Cas9 technology. The main goal will be the construction of knockout generation of Chlamydomonas reinhardtii strain in genes involved in biosynthesis of secondary metabolites. Subsequently, use the ambient mass spectrometry under ambient conditions with desorption electrospray ionization (DESI) and direct analysis in real time (DART) to study metabolome in the obtained strains. 

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Novel technology platforms for bioanalysis using magnetic nano- and microparticles

Nowadays, analytical techniques do not benefit only from expensive benchtop devices, which are suitable for laboratory use only. Technological progress enables to integrate several simple elements such as miniaturized electrodes, LED diodes, computers, and other necessary hardware platforms into simple and cheap bioanalytical devices. The key tool in this field seems to be fused deposition modelling based 3D printing and designed magnetic micro and nanoscale particles. Mentioned magnetic materials often possess spherical shape and surface modification suitable for selective target molecule isolation/preconcentration. The aim of dissertation is to design and fabricate portable device, which can be controlled by Bluetooth of cell phones. Student will acquire skills in the field of chemical analysis, 3D printing, magnetic materials synthesis, modification and characterization (SEM, TEM, FTIR, XPS, DLS…). The special attention will be paid the isolation of nucleic acids. Further, the student will acquire experience from prototype fabrication and its testing.

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