Standard crystallization Screen Set-up, Plate Storage and Inspection


Technology / Methodology:
Crystallization of biomolecules

Set-up of crystallization screens (drop volume 200-300 nl) using commercial kits (including kits for membrane proteins, protein complexes and nucleic acids). Sitting drop crystallization method, crystallization under oil. } Storage of the screening plates for 3 months at a constant temperature 4°C or 20°C Automatic screening of crystals . Regular automatic inspection within the period of one month. UV imaging to distinguish a protein from a salt. Online access to the images. Storage of the screening plates for extended period of time (6 months) at a constant temperature 4°C or 20°C with a possibility of a demanded extra inspection


Standard crystallization Screen Set-up, Plate Storage and Inspection