1. Mass spectrometric imaging of biological tissues
Supervisor: prof. Jan Preisler
Consultant: Mgr. Markéta Machálková
Annotation: Mass spectrometry imaging (MSI) exploits the ability of mass spectrometry to identify molecules according to their specific mass and image their distribution across the sample. The method allows direct visualization of endogenous compounds (lipids, proteins, saccharides) as well as exogenous drugs and their metabolites. The aim of the research will be development of a suitable matrix-assisted laser desorption/ionization (MALDI) MSI method for the study of distribution and the effect of chosen drug selected tissues and cell lines. Special attention will be devoted to sample preparation and matrix deposition (spray, sublimation, piezoelectric dispenser). The samples will be supplied by cooperating institutions (e.g. the Department of Experimental Biology, Experimental and Applied Neuropsychopharmacology, Centre for Neuroscience, the Department of Pharmacology, the Regional Centre for Applied Molecular Oncology). The analyzed compounds include endogenous compounds (lipids, glycans) as well as selected drugs.
RG: Jiří Pinkas Research Group
Keywords: mass spectrometry imaging, MSI, matrix-assisted laser desorption/ionization,MALDI, analysis development