Zeiss AxioZoom.V16-Apotome2 (AxioZoom.V16-Apotome)


Guarantor: Milan Ešner, Ph.D.
Instrument status: Operational Operational, 28.5.2020 13:08
Equipment placement: CEITEC MU : A2 -1.15
Research group: CF: Cellular Imaging Core Facility

Detailed description:

Fully motorized fluorescence stereo zoom microscope for large fields AxioZoom.V16 combines a 16x zoom with a high numerical aperture objectives. With Plan-NEOFLUAR Z 2.3x you achieve a numerical aperture of NA 0.5 in an object field of 1.5 millimeters. You can select transmitted, reflected mode or fluorescence mode together with structured illumination module Apotome.2 to perform optical sections with high contrast on thick specimens.

Fluorescence light source: HXP 120V.

Filters for fluorescence: DAPI (F-set 96HE), GFP (F-set 38), mcherry (F-set 63HE), (on demand: CFP (F-set 47), Cy5 (F-set 50)).

Cameras: color camera AxioCam 305 color, 2464 x 2056 pixels, 3.45 x 3.45 μm size; monochromatic camera AxioCam 512 mono, 4250 x 2838 pixels, 3.1 x 3.1 μm size.

Objectives: Plan Neofluar 1x / 0.25 fwd 56 mm, Plan Neofluar 2.3x / 0.57 fwd 10.6 mm.

Transmitted light source: CL 9000 LED CAN.

Transmitted light techniques: Brightfield, Darkfield, Oblique illumination.

Software: ZEN Blue.