Q-Phase holographic microscope (Q-Phase)


Guarantor: Zbyněk Dostál, Ph.D.
Instrument status:
Equipment placement: Vysoké učení technické v Brně, CEITEC
Research group: Experimental Biophotonics - Radim Chmelík

Detailed description:

Multimodal holographic microscope (MHM) uses incoherent holography, which enables to acquire quantitative phase images with high lateral resolution comparable with conventional widefield microscopes.

Featured operating modes
- Transmission mode: suitable for biological and biotechnical applications
(cell life cycle, cell growth, cell motility, morphological observations, etc.).
- Reflection mode: suited for applications from material science and topography studies with nanometer axial
- Fluorescence mode: enables additional comparative experiments (DAPI, FITC, Texas Red)

Advantages of MHM
- Quantitative phase imaging (without halo effect)
- imaging of live cells in their native environment
- label-free, non-invasive observation
- observation in turbid media
- system located in incubator (adjustable temperature)
- imaging without speckles and parasitic interferences (in comparison with laser-based HM)
- fast image acquisition (no scanning necessary)
- user-friendly system: motorized parts (objective exchange, sample stage and focusing), automated microscope
adjustment, easy operating with control panel, automated multidimensional acquisition (time-lapse, multichannel,