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The Advanced Light Microscopy Methods is an advanced course for students, postdocs, technicians, and other laboratory staff in biology and biomedical science who require specialised microscopy methods for their experiments. The course consists of theoretical lectures (25%) and practical hands-on sessions on selected microscopes (50%) and image processing software (25%).
Overall, this course provides an overview of core principles behind super-resolution, FLIM-FRET and mesoscopic imaging methods, together with corresponding essential image processing steps. Participants will learn theoretical principles behind large sample imaging, super-resolution methods like Airyscan, Optical Reassignment, Structured Illumination and Single Molecule Localisation, as well as Fluorescence Lifetime and Förster Resonance Energy Transfer Microscopy.
A dedicated practical session will demonstrate the learned principles directly on real microscope acquisitions. Participants will gain skills needed to operate selected microscopes and experience how to setup advanced experimental workflow, and perform the most common troubleshooting.
The workshop concludes with an overview of related workflows in image processing and analysis utilising related software (ZEN, LAS X). Finally, the participants will receive comprehensive guidelines for advanced analysis and image visualisation in Imaris.
Practical training will be conducted on the following systems:
Carl Zeiss LSM880 – Airyscan
Carl Zeiss Elyra 7 – SIM/SMLM
Leica Stellaris 8 – FALCON FLIM
Carl Zeiss Lightsheet 7 – SPIM
Image processing and analysis software – ZEN, LAS X, Imaris
More information
PARTICIPANTS: maximum 12
LEVEL/PREREQUISITE: Microscopists with prior experience in standard microscopy techniques (Previous level: Fundamentals of Light Microscopy)
COSTS (including coffee breaks and refreshments):
MUNI Academic: 2, 300 CZK + VAT,
External Academic: 3, 300 CZK + VAT,
Commercial: 7, 000 CZK + VAT
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