Confocal microscope Nikon A1R (Konfokál)

CONTACT US

Guarantor: Alena Vojkůvková
Instrument status:
Equipment placement: Vysoké učení technické v Brně, FSI
Research group: Experimental Biophotonics - Radim Chmelík


Detailed description:

Observation techniques:
- Confocal scanning mode
- Bright field
- Reflection interference contrast (RICM)
- Differential interference contrast (DIC)
- Epi-fluorescence
+ Perfect Focus System

Objectives:
- PLAN APOCHROMAT VC 20X N.A. 0.75 (lateral resolution = 0.3 um, axial resolution = 1.8 um)
- PLAN APOCHROMAT VC 60X WI N.A. 1.2 (lateral resolution = 0.18 um, axial resolution = 0.7 um)

Field of view:
635x635 um for 20X objective
205x205 um for 60X objective

Light sources:
- Diode laser (640 nm)
- Multiline argon laser (457/477/488/514 nm)
- Halogen lamp

Fluorescent cubes:
- DAPI
- FITC
- TRITC

Scanners:
- Galvo scanner 4 fps (512 x 512 pixel),
- Resonant scanner 30 fps (512 x 512 pixel) to 230 fps (512 x 64 pixel), 15 600 lines/sec (line speed)

Piezo stage:
- maximal range 100 um
- resolution 0.2 nm
- maximal load 0,5 kg

Detectors:
- standard fluorescence detector (400-750 nm)
- diascopic detector (450-650 nm)
- monochromatic camera ProgRes MF (1.4 MPix 2/3´´ CCD, 1360x1024, 12bit, IR-cut-off)

Software: NIS-Elements C, V4.10