Course: Fundamentals of Light Microscopy

27. 4. - 28. 4. 2020, 09:30 - 17:00
CELLIM, CEITEC, University Campus Bohunice

We would like to invite you to a course organized by CELLIM Core Facility:

Fundamentals of Light Microscopy

This course is intended for students who would like to learn about the fundamentals and principles of light microscopy. During the course participants will learn how image is formed in microscope, what are the principle parts of microscope, how to prepare samples and how to properly acquire image using widefield and confocal system. We will also touch basics of image processing, mainly how to work with channels, add scale bar and do the simple measurements.

The course is free of charge, but there is limited number of places.


April 27 - 28, 2020


April 27, 2020  A35/211
9:30 – 12:00    
Welcome, principles of light microscopy, properties of light, transmitted light microscopy, different contrasting techniques DIC, phase contrast, dark field, fluorescence.

13:30 – 17:00   
Widefield microscopy, optical sectioning methods, confocal microscopy, sample preparation and labelling.

April 28, 2020   A2/CELLIM
9:30 – 13:00    
Practical: Image acquisition at different microscopy platforms – widefield and confocal microscopes.

April 28, 2020   A35/211
14:30 – 17:00
Practical: Basics of image processing and analysis.


Registration has been closed.