Course: Fundamentals of Light Microscopy
Fundamentals of Light Microscopy
This course is intended for students who would like to learn about the fundamentals and principles of light microscopy. During the course participants will learn how image is formed in microscope, what are the principle parts of microscope, how to prepare samples and how to properly acquire image using widefield and confocal system. We will also touch basics of image processing, mainly how to work with channels, add scale bar and do the simple measurements.
The course is free of charge, but there is limited number of places.
April 27 - 28, 2020
April 27, 2020 A35/211
9:30 – 12:00
Welcome, principles of light microscopy, properties of light, transmitted light microscopy, different contrasting techniques DIC, phase contrast, dark field, fluorescence.
13:30 – 17:00
Widefield microscopy, optical sectioning methods, confocal microscopy, sample preparation and labelling.
April 28, 2020 A2/CELLIM
9:30 – 13:00
Practical: Image acquisition at different microscopy platforms – widefield and confocal microscopes.
April 28, 2020 A35/211
14:30 – 17:00
Practical: Basics of image processing and analysis.