Workshop Registration

Multimodal Microscopy Workshop: Probing the Triad of Structure, Mechanics, and Chemistry in Biological Systems

About event

Use of Atomic Force Microscopy, Raman microscopy, and Fluorescence Microscopy, while emphasizing the investigation of structure, mechanical properties, and chemical composition in biological samples.

Please register via the registration form. Don't forget to pay the workshop fee at the Shopping Center MUNI. Links are below.

Workshop fee

Programme

Monday, June 10th 

8:30–9:00 Registration, coffee (E35, Atrium) 
9:00–9:15 Workshop opening (E35, Atrium) 
9:15–9:40 Company presentation, J. Horák, MTM (E35, Atrium) 
9:40–10:10 AFM introduction and probe selection guide, L. Pařízek, MTM (E35, Atrium) 
10:10–10:30 Coffee break (E35, Atrium) 
10:30–11:30 Invited talk: Biomechanics and biorheology – their relation to a cell diseased state, Małgorzata Lekka, Department of Biophysical Microstructures, Polish Academy of Sciences, Krakow, Poland (E35, Atrium) 
11:30–12:30 Lunch (E35, Atrium) 
12:30–12:50 Mechanics by AFM, A. Dulebo, Bruker (E35, Atrium) 
12:50–13:10 AFM with other techniques, A. Dulebo, Bruker (E35, Atrium) 
13:10–13:30 High-speed AFM, A. Dulebo, Bruker (E35, Atrium) 
13:30–14:00 AFM data processing, Š. Klimovič, CEITEC MU (E35, Atrium) 
14:00–14:30 Coffee break (E35, Atrium) 
14:30–15:00 Core Facility Nanobiotechnology, J. Přibyl, CEITEC MU (E35, Atrium) 
13:00–17:30

Sample / user stories (E35, Atrium)

  • Electromechanical cell-based biosensor for drug and disease of 3D cardia models , Martin Pešl, Masaryk University and ICRC-FNUSA, Brno, Czech Republic. 
  • Correlative analysis of biomechanical properties and morphology on connective tissue and significance for fascia, Daniel Hadraba, Institute of Physiology, CAS, Prague, Czech Republic.) 
  • Detection of oncomarkers by AFM, Michaela Domšicová, CBs SAS, Bratislava, Slovakia. 
  • Characterization of surface and mechanical properties of microbial cells, Markéta Khýrová, Faculty of Chemistry, Brno University of Technology and Institute of Scientific Instruments, Brno, Czech Republic. 
  • Investigation of Mechanisms Underlying Stiffness Change in Prostate Cancer Cells Using AFM Computational Simulations, Lucie Jadrna, Brno University of Technology, Brno, Czech Republic. 
  • TBA, Astrid Laimer-Digruber, University of Veterinary Medicine, Vienna, Austria 

Tuesday, June 11th 

8:30–9:00 Getting together, coffee (E35, Atrium) 
9:00–9:30 Specific approaches in microscopy samples preparation, J. Přibyl, CEITEC MU (E35, Atrium) 
9:30–10:30 How to prepare biological samples for AFM measurements, Małgorzata Lekka, Department of Biophysical Microstructures, Polish Academy of Sciences, Krakow, Poland (E35, Atrium) 
10:30–10:45 Coffee break (E35, Atrium) 
10:45–11:00 Cytotoxicity of nanoparticles released from titanium implants, Jakub Hruška, CEITEC MU and Faculty of Medicine, Masaryk University, Brno, Czech Republic (E35, Atrium) 
11:00–11:30 TBA, Delong Instruments a Brnoregion Microscopy Community(E35, Atrium) 
11:30–12:00 Quick demonstration of instruments
12:00–13:00 Lunch (E35, Atrium) 
13:00–17:30

Afternoon practical session (E35, CF labs)

  • Bruker TS77 and Biosoft – Mechanical testing of hydrogels (E35, 2S015) 
  • Bruker JPK NanoRacer HS-AFM – Observing molecular dynamics using AFM (E35, 2S012) 
  • Bruker Fastscan – Membrane testing (E35, 2S014) 
  • JPK Nanowizard 3 + Olympus FluoView 1200 - Combination of AFM with confocal fluorescence – application on cells (E35, 2S012) 
  • Renishaw InVia Raman microscope – combination of optical and Raman microscopy – study of microplastic contamination (E35, 2S014) 
19:00–22:30 Workshop dinner

Wednesday, September 12th 

8:30–9:00 Getting together, coffee (E35, Atrium) 
9:00–12:00

Practical session (E35, CF labs) 

  • Bring your own sample 
  • Hands-on practice 
12:00–13:00 Lunch (E35, Atrium) 
13:00–13:30 Closing remarks (E35, Atrium) 

 

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More information

Speakers will be submitting an abstract for their talk. Download the abstract template HERE.

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Date

10. - 12. 6. 2024
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Organizer

Nanobiotechnology Core Facility
Nanobiotechnology Core Facility
CEITEC MU

Venue

CEITEC MU, University Campus, Building E35, Atrium