Nowadays, Open Science is a hot topic on the national and international level. It is not only about Open Access but involved also Open Research Data, Open Research Infrastructure or Citizen Science. It is widely recognised that making research results more accessible contributes to better and more efficient science, and to innovation in the public and private sectors. CEITEC participates in Horizon 2020 project ORION Open Science which aims is building of effective cooperation between science and society. This Open Science Guide will provide you an overview of themes, events, and news related to Open Science.