About event
Imagine you could directly see the location and dynamics of individual proteins binding to a single piece of DNA or RNA in real time. What if you could hold a single protein or nucleic acid and manipulate its structure to interrogate its conformational landscape? What if you could assemble your biological complex step by step and expose it to different buffer conditions to test your experimental hypotheses?
With the LUMICKS C-Trap, the world’s first dynamic single-molecule microscope combining high-resolution optical tweezers, fluorescence microscopy, and advanced microfluidics in a truly integrated system, you can do all of this! We will illustrate how the dynamic single-molecule approach can shed light on a multitude of biological processes: from the mechanism of action of nucleic acid binding enzymes to protein folding and conformational changes, from chromatin remodelling to properties of molecular condensates.
These experiments show that technological advances in hybrid single-molecule methods can be turned into an easy-to-use and stable instrument enabling control, visualization and manipulation of single molecules in real time. This gives researchers the power to directly prove molecular mechanisms, in ways not previously possible, allowing you to answer mechanistic questions faster.
After the session, there will be a possibility to discuss the user’s sample. Please contact us (bic@ceitec.cz) to reserve a slot.