About event
This seminar will present a new rapid and robust method for single molecule analysis of DNA binding proteins from nuclear extracts (SMADNE) of human cells expressing a fluorescently tagged protein of interest (Schaich et al., Nucleic Acids Res. 2023 Apr 24;51(7):e39). SMADNE when combined with the LUMICKS C-trap provides unprecedented observations of DNA repair protein dynamics on damaged DNA. Over the last 18 months our group has been able to analyze the dwell times of 35 proteins or protein variants on DNA substrates using this approach. After a brief introduction to DNA damage and base excision repair, this seminar will discuss new unpublished data, including the DNA binding dynamics of PARP1 and PARP1 variants or DNA LIG3-XRCC1 on nicks in naked DNA and in a nucleosomes. An important glycosylase, TDG, involved in active oxidative demethylation of 5mC through base excision repair will also be discussed (Nat Commun. 2024 Oct 25;15(1):9226.) Finally, the non-specific DNA interactions and the binding behavior to 8-oxoG of purified eGFP-OGG1, purified eGFP-OGG1 added to extracts, and eGFP-OGG1 from human cell nuclear extracts will be directly compared.
The lecture is part of the Structural Biology Seminar Series. PhD candidates, postdocs, and research group leaders from the structural biology field present their newest research results to the MUNI scientific community. Ideal space for knowledge sharing, networking and professional growth.
