Workshop

Super resolution microscopy based on spinning-disc and optical photon reassignment

About event

Spinning-disk confocal microscopy is a proven technology for investigating 3D structures of biological specimens. With the help of a newly modified Nipkow disk (SoRa), which comprises pinholes and micro-lenses on the front and back, respectively, emitted photons from the specimen can be optically reassigned to the most probable locations they originate from. Consequently, the improvement in resolution is achieved preserving the inherent sectioning capabilities of confocal microscopy.

Data reliability is ensured owing to our Olympus Super Resolution (OSR) technology. Based on optical theory, our OSR processing algorithms are capable of obtaining a spatial resolution of 120 nm, providing highly reliable results below the diffraction limit. Deep tissue observation is achieved using silicone immersion objectives and through the optical sectioning capability of confocal microscopes.

Programme

6 November 2023

10:00-11:00 Welcome & Introduction, Tomáš Pop, Evident Room C02/211
13:00-17:00 Hands-on Session Room C02/113

7-10 November 2023

9:00-17:00 Hands-on Session Room C02/113

 

More information

Hands-on registration: tomas.pop@evidentscientific.com

 

Date

6. - 10. 11. 2023
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Organiser

CF: Cellular Imaging Core Facility
CF: Cellular Imaging Core Facility
CEITEC MUNI

Venue

CEITEC MU, University Campus, Building C02, Room 211 + 113