About event
Lectures
Jan Přibyl (CEITEC MU):
AFM microscopy and spectroscopy – basic and advanced imaging modes
Practical session with JPK NanoWizard3.
Matěj Pastucha (CEITEC MU):
AFM data analysis.
Practical session with Bruker Dimension FastScan.
Šimon Klimovič (CEITEC MU):
Preparation of samples for AFM.
Processing of AFM mechanical data.
Practical session with JPK NanoWizard3.
Hands-on session
Collagen fibres characterization by hybrid AFM mode (PF-QNM).
Living cells imaging and mechanical mappping.
Bruker Dimension FastScan
JPK NanoWizard3
Preliminary schedule
Monday 9th September
10:00 Meet and Greet by coffee machine / Seminar room 211
10:15-11:45 Lecture: Sample Preparation for AFM Microscopy, Matěj Pastucha, Šimon Klimovič (CEITEC MU)
11:50-12:35 Lecture: Advanced modes of Atomic Force Microscopy, Jan Přibyl (CEITEC MU)
13:45-14:00 Introduction to Nanobio Core Facility Labs and Equipment / NanoBioCF Labs 2S015, 2S014, 2S012.
14:00-15:00 Hands-on: Sample Preparation - Immobilization of Collagen Fibres on Mica, Matěj Pastucha, Šimon Klimovič (CEITEC MU) / NanoBioCF Lab 2S014
15:00-15:15 Coffee break
15:15-17:00 Hands-on: PF-QNM Mode for Characterization of Collagen Fibres, Matěj Pastucha, Jan Přibyl (CEITEC MU) / Bruker Dimension FastScan AFM microscope / NanoBioCF Lab 2S014
Tuesday 10th September
8:45 Meet and Greet by coffee machine
9:00 - 11:30 Hands-on: Complex Characterization of Living Cells in QI and Force-Mapping Mode, Jan Přibyl, Šimon Klimovič (CEITEC MU) / JPK NanoWizard3 / NanoBioCF Lab 2S015
11:30 - 12:30 Lecture & Hands-on: Gwyddion Software for Processing and Presentation of AFM Data, Jan Přibyl (CEITEC MU) / Bring your laptop – to follow the speaker and process your data.
13:30 - 16:00 Lecture & Hands-on: Processing of AFM Mechanical Data – JPK DP, AtomicJ Software, Jan Přibyl, Šimon Klimovič (CEITEC MU) / Bring your laptop – to follow the speaker and process your data.
16:00 - 16:20 Closing remarks.
Please register at: pribyl@nanobio.cz