Workshop Registration

Advanced light microscopy methods in biology

About event

We would like to invite you to a practical course on advanced methods in light microscopy. The course will cover different methods of super resolution imaging (Image Scanning Microscopy, SIM and STORM), expansion microscopy and light sheet microscopy, including sample preparation. The theoretical part will be followed by a hands-on sessions where you will have the opportunity to try out different techniques on real samples, including session for image analysis and visualisation. The course is primarily aimed at students who already have experience of standard microscopy techniques.

Places are limited. Registration is required - see the link below. Successful candidates will be selected on a first come first served basis. Payment details will be sent to participants at the time of their selection.

Registration fee:

  • 1000 CZK + VAT - MUNI academic users,
  • 3000 CZK + VAT - external academic users,
  • 6000 CZK + VAT - commercial users.

Coffee and refreshments will be provided during the course.

Programme

Day 1, September 10: C02/121

9:00–17:00

Theoretical lectures

  • Milan Esner: Diffraction limits in light microscopy and approaches to improve the resolution
  • Jakub Pospisil: Single molecule localization microscopy - STORM; Expansion microscopy
  • Sona Legartova: Light-sheet microscopy and imaging of large specimens; Sample preparation for lightsheet microscopy
  • Narendra Varma Gottumukkala: SIM microscopy from user perspective

Day 2, September 11: C02/CELLIM

9:00–12:30

Practical 3 x groups

  1. Airyscan
  2. SIM/SMLM
  3. Lightsheet
13:30–17:00

Practical 3 x groups

  1. Airyscan
  2. SIM/SMLM
  3. Lightsheet

Day 3, September 12: C02/CELLIM

9:00–13:00

Practical 3 x groups

  1. Airyscan
  2. SIM/SMLM
  3. Lightsheet
14:00–17:00 Wojciech Jesionek, Jakub Pospisil: Image processing and analysis

 

Advanced light microscopy methods in biology

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Date

10. - 12. 9. 2024
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Organizer

CF: Cellular Imaging Core Facility
CF: Cellular Imaging Core Facility
CEITEC MUNI