Confocal microscope Nikon A1R (Konfokál Nikon A1R)
Guarantor:
Alena Vojkůvková
Instrument status:
Operational, 2.8.2023 11:10
Equipment placement:
Vysoké učení technické v Brně, FSI
Research group:
Experimental Biophotonics
Description:
.Observation techniques:
Confocal scanning mode
Bright field
Reflection interference contrast (RICM)
Differential interference contrast (DIC)
Epi-fluorescence
+ Perfect Focus System
Objectives:
PLAN APOCHROMAT VC 20X N.A. 0.75 (lateral resolution = 0.3 um, axial resolution = 1.8 um)
PLAN APOCHROMAT VC 60X WI N.A. 1.2 (lateral resolution = 0.18 um, axial resolution = 0.7 um)
Field of view:
635x635 um for 20X objective
205x205 um for 20X objective
Light sources:
Diode laser (640 nm)
Multiline argon laser (457/477/488/514 nm)
Halogen lamp
Fluorescent cubes:
DAPI, FITC, TRITC
Scanners:
Galvo scanner 4 fps (512 x 512 pixel),
Resonant scanner 30 fps (512 x 512 pixel) to 230 fps (512 x 64 pixel), 15 600 lines/sec (line speed)
Piezo stage:
maximal range 100 um
resolution 0.2 nm
maximal load 0,5 kg
Detectors:
standard fluorescence detector (400-750 nm)
diascopic detector (450-650 nm)
monochromatic camera ProgRes MF (1.4 MPix 2/3´´ CCD, 1360x1024, 12bit, IR-cut-off)
