Differential scanning fluorimetry - NanoTemper Prometheus (nanoDSF)
CONTACT US
Guarantor:
Eva Paulenová, Ph.D.
Technology / Methodology:
Interakce a charakterizace biomolekul
Instrument status:
Operational, 9.3.2020 14:35
Equipment placement:
Masarykova univerzita, UKB
Research group:
Biomolecular Interactions and Crystallography Core Facility
Description:
The device is used to determine thermal unfolding transition temperatures and denaturation midpoints. Prometheus uses advanced UV-LEDs for excitation of fluorophores. Proprietary UV-detection unit with the ability of rapid and sensitive data acquisition detects changes in fluorescence of the amino acid fluorophores (mainly tryptophan) over wide range of temperatures.