Fundamentals of Light Microscopy

About event

We would like to invite you to a course organized by CELLIM Core Facility:

Fundamentals of Light Microscopy

This course is intended for students who would like to learn about the fundamentals and principles of light microscopy. During the course participants will learn how image is formed in microscope, what are the principle parts of microscope, how to prepare samples and how to properly acquire image using widefield and confocal system. We will also touch basics of image processing, mainly how to work with channels, add scale bar and do the simple measurements.

The course is free of charge, but there is limited number of places.


November 8–⁠10, 2021


Monday, 8/11/2021
Online Lectures ZOOM
  • Fundamentals of Light Microscopy
  • Contrasting Techniques in Transmitted Light Microscopy: Brightfield, Darkfield, Phase Contrast, DIC
  • Widefield Fluorescence Microscopy
  • Confocal Microscopy - Principles, Types of Confocal Microscopes
  • Lightsheet Microscopy
14:00–17:00 Imaging Below Diffraction - Principles of Super-resolution Microscopy
Tuesday, 9/11/2021
Onsite C02/CELLIM
9:00–17:00 Practical Training: Widefield, Confocal, Lightsheet, Super-resolution Microscopy
Wednesday, 10/11/2021
Online Lectures and Practicals ZOOM
9:00–12:00 Basics of Image Analysis: Image Types, Pixels, Voxels, Basic Filters, Image Processing, Metadata, Deconvolution, 3D Visualization, Objects Detection, Quantification
14:00–17:00 Practical Examples Using ZEN, IMARIS, FIJI, CellProfiler



8. - 10. 11. 2021
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