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Home Gel Permeation Chromatography (GPC)/Size Exclusion Chromatography (SEC) with Multi-Angel Light Scattering (MALS)

Gel Permeation Chromatography (GPC)/Size Exclusion Chromatography (SEC) with Multi-Angel Light Scattering (MALS) (DAWN-HELEOS)

Gel Permeation Chromatography (GPC)/Size Exclusion Chromatography (SEC) with Multi-Angel Light Scattering (MALS)
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Guarantor: M.Eng. Lizeth Katherine Tinoco Navarro
Instrument status: Operational Operational, 23.7.2024 10:55
Equipment placement: CEITEC Nano - B1.15
Research group: CF: CEITEC Nano

Description:

Gel Permeation Chromatography (GPC), also known as Size Exclusion Chromatography (SEC), is an instrumental preparative analytical technique designed for the separation of polymer and biopolymers by their molecular size. Absolute molecular weight is measured using multi-angle light scattering (MALS) detection and RI detector. NECESSARY CONDITION: Solubility of tested (bio)polymers in organic solvents or aqueous buffers.

Publications:

  • KADLECOVÁ, Z.; SEVRIUGINA, V.; LYSÁKOVÁ, K.; RYCHETSKÝ, M.; CHAMRADOVÁ, I.; VOJTOVÁ, L., 2023: Liposomes Affect Protein Release and Stability of ITA-Modified PLGA-PEG-PLGA Hydrogel Carriers for Controlled Drug Delivery. BIOMACROMOLECULES 25(1), p. 67 - 10, doi: 10.1021/acs.biomac.3c00736; FULL TEXT
    (DAWN-HELEOS, ZETASIZER, NMR, MIRA-STAN)