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About event
Sample quality is important step for obtaining good quality data for subsequent techniques and to be able to reproduce your results. However, chcking of sample purity by SDS.PAGE shloud not be the only quality control you do! As a minimum, you should check homogenity of your sample (DLS, AUC), proper folding (CD), thermal stability, optimize buffer (DSF, DSC, CD) and determine oligomerit state of your sample (AUC).
Preliminary program
May 23
building A35, room 211
12:00 – 12:30 Registration
12:30 – 12:45 Introduction (Michaela Wimmerová)
12:45 – 13:30 Introduction to sample quality + DSF (Bertrand Raynal)
13:30 – 14:15 AUC sedimentation analysis (Ondřej Vaněk)
14:15 – 14:45 Sample quality at VBCF, Vienna (Arthur Sedivy)
14:45 – 15:30 Coffee break
15:30 – 16:00 Sample characterization – CD (Arthur Sedivy)
16:00 – 16:45 DSC for characterization of protein stability (Margarida Bastos)
16:45 – 17:30 Importance of sample quality (Stefan Knauer)
17:30 – 18:00 Discussion and closing remarks
May 24
building A4, room 218 + 223
Group A Group B
09:30 – 11:30 session 1 CD AUC
11:30 – 12:30 lunch (not included)
12:30 – 14:30 session 2 AUC CD
14:30 – 15:00 coffee break
15:00 – 16:30 session 3 DSF DLS
16:30 – 18:00 session 4 DSC AUC evaluation
May 25
building A4, room 218 + 223
10:00 – 11:30 session 5 DLS DSF
11:30 – 12:30 lunch (not included)
12:30 – 14:00 session 6 AUC evaluation DSC
14:00 – 15:00 DSC evaluation and summary of all techniques (both groups - A35/211)
Tutors (practical part) + room number:
AUC – Jan Komárek – A4/217
AUC evaluation – Jan Komárek – A4/1S57
CD, DLS – Josef Houser – A4/223
DSF – Jitka Ždánská – A4/221
DSC – Josef Houser – A4/218
Registration form is HERE. The deadline for registration is May 15. For more information contact us at bic@ceitec.cz.