How to Characterize Your Sample and Check Its Quality: Handbook for Life-scientist

23. 5. - 25. 5. 2018

Sample quality is important step for obtaining good quality data for subsequent techniques and to be able to reproduce your results. However, chcking of sample purity by SDS.PAGE shloud not be the only quality control you do! As a minimum, you should check homogenity of your sample (DLS, AUC), proper folding (CD), thermal stability, optimize buffer (DSF, DSC, CD) and determine oligomerit state of your sample (AUC).

 

 

 

 

Preliminary program 

May 23

building A35, room 211

12:00 – 12:30 Registration

12:30 – 12:45 Introduction (Michaela Wimmerová)

12:45 – 13:30 Introduction to sample quality + DSF (Bertrand Raynal)

13:30 – 14:15 AUC sedimentation analysis (Ondřej Vaněk)

14:15 – 14:45 Sample quality at VBCF, Vienna (Arthur Sedivy)

14:45 – 15:30 Coffee break

15:30 – 16:00 Sample characterization – CD (Arthur Sedivy)

16:00 – 16:45 DSC for characterization of protein stability (Margarida Bastos)

16:45 – 17:30 Importance of sample quality (Stefan Knauer)

17:30 – 18:00 Discussion and closing remarks

 

May 24

building A4, room 218 + 223

                                                         Group A            Group B

09:30 – 11:30 session 1                        CD                     AUC

11:30 – 12:30 lunch (not included)

12:30 – 14:30 session 2                        AUC                   CD

14:30 – 15:00 coffee break

15:00 – 16:30 session 3                        DSF                    DLS

16:30 – 18:00 session 4                        DSC            AUC evaluation


May 25

 building A4, room 218 + 223

10:00 – 11:30 session 5                        DLS                    DSF

11:30 – 12:30 lunch (not included)

12:30 – 14:00 session 6                 AUC evaluation        DSC

14:00 – 15:00 DSC evaluation and summary of all techniques (both groups - A35/211)

Tutors (practical part) + room number:

AUC – Jan Komárek – A4/217
AUC evaluation – Jan Komárek – A4/1S57
CD, DLS – Josef Houser – A4/223
DSF – Jitka Ždánská – A4/221
DSC – Josef Houser – A4/218


Registration form is HERE. The deadline for registration is May 15. For more information contact us at bic@ceitec.cz.