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About Event
In collaboration with Anamet and Specion we are pleased to invite you to participate in an advanced training course focused on key biophysical techniques for studying biomolecular structure, stability, and interactions.
The aim of this course is to provide a comprehensive understanding of the principles and practical applications of Isothermal Titration Calorimetry (ITC), Differential Scanning Calorimetry (DSC), Circular Dichroism (CD) and Fluorescence Spectroscopy (FS). Through a combination of theoretical lectures and hands-on laboratory sessions, attendees will develop experimental competence, strengthen analytical thinking, and gain confidence in data interpretation. By the end of the course, participants will be equipped to apply these methods effectively in their own research projects.
The program will include lectures open to a broader audience, while practical sessions will be reserved for registered participants to ensure maximum hands-on experience. Participants will have the opportunity to measure their own samples upon discussion.
The practical part will be carried out on the instruments PEAQ-DSC Automated (Malvern), PEAQ-ITC Automated (Malvern), Chirascan V100 (Applied Photophysics), Fluorolog QM and Duetta (HORIBA).
Upon registration you will be asked to send CV and motivation letter - 16 participants for practicals will be selected according to specific criteria (carrier stage, institutional location, potential for know-how transfer, etc.) . The selected participants willbenefit from reimbursement of 200 €/person.
Deadline for registration to the whole workshop is 19th July 2026 - The course is already full, you can now register only for lectures.
Speakers:
Tomáš Brom (NCBR Masaryk University, CZ)
Josef Houser (CEITEC, CZ)
Hssan Fares (Horiba)
Raul Pacheco Gomez (Malvern, UK)
Grzegorz Piszczek (NIH - NHLBI, USA) - online
Peter W. Schuck (NIH - NIBIB, USA) - online
Martin Textor (Applied Photophysics, GE)
Huaying Zhao (NIH - NIBIB, USA) - online
Programme
Day 1:
12:30 – 13:00 Registration
13:00 – 13:10 Welcome
13:10 – 14:40 Lecture: Biomolecules and their interactions (Josef Houser)
14:40 – 15:00 Coffee break
15:00 – 16:00 Lecture: Sample quality assessment (Josef Houser)
16:00 – 17:00 Initial discussion of participants' scientific background and projects
Day 2:
09:00 – 10:30 Lecture: Biomolecular calorimetry – Introduction ITC (Raul Pacheco Gomez)
10:30 – 11:00 Coffee break
11:00 – 12:30 Lecture: Biomolecular calorimetry – Introduction DSC (Raul Pacheco Gomez)
12:30 – 12:35 Group photo
12:35 – 13:30 Lunch break
13:30 – 15:00 Hands-on: Isothermal titration calorimetry/Differential scanning calorimetry (Raul Pacheco Gomez/Jitka Holková)
15:00 – 15:30 Coffee break
15:30 – 17:00 Hands-on: Isothermal titration calorimetry/Differential scanning calorimetry (Raul Pacheco Gomez/Jitka Holková)
17:00 – 18:00 Hands-on: Calorimetrical measurement of attendees’ samples (optional)
Day 3:
09:00 – 10:30 Lecture: Biomolecular calorimetry – Applications ITC (Raul Pacheco Gomez)
10:30 – 11:00 Coffee break
11:00 – 12:30 Lecture: Biomolecular calorimetry – Applications DSC (Raul Pacheco Gomez)
12:30 – 13:30 Lunch break
13:30 – 15:30 Hands-on: Calorimetry data evaluation (Raul Pacheco Gomez)
15:30 – 16:00 Coffee break
16:00 – 17:30 Hands-on: Complex data analysis using SEDPHAT (Peter Schuck, Grzegorz Piszczek, Huaying Zhao )
17:30 – 18:30 Tour of CEITEC MU Core Facilities (optional)
18:30 – 21:00 Social event (optional)
Day 4:
09:00 – 10:30 Lecture: Biomolecular fluorescence spectroscopy (Hssan Torres)
10:30 – 11:00 Case study: Novel approach to FRET measurement set-up and analysis (Tomáš Brom)
11:00 – 11:15 Coffee break
11:15 – 12:45 Lecture: Circular dichroism spectroscopy (Martin Textor)
12:45 – 13:45 Lunch break
13:45 – 14:45 Lecture: Circular dichroism spectroscopy – applications (Martin Textor)
14:45 – 15:00 Coffee break + move to C04
15:00 – 16:30 Hands-on: Circular dichroism (Martin Textor)/ Fluorescence spectroscopy Duetta (Hssan Fares)
16:30 – 18:00 Fluorescence spectroscopy Duetta (Hssan Fares)/ Fluorolog QM ((Jitka Holkova)
Day 5:
09:00 – 10:30 Hands-on: Circular dichroism (Martin Textor)/ Fluorolog QM ((Jitka Holkova)
10:30 – 10:45 Coffee break
10:45 – 12:00 Hands-on: CD spectroscopy data evaluation (Martin Textor)
12:00 – 12:30 Final discussion and course wrap-up
12:30 – 13:30 Lunch break
13:30 – 15:30 Hands-on: Spectroscopic measurement of attendees’ samples (optional)
For more information contact bic@ceitec.cz
