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About event
In collaboration with Anamet and Specion we are pleased to invite you to participate in an advanced training course focused on key biophysical techniques for studying biomolecular structure, stability, and interactions.
The aim of this course is to provide a comprehensive understanding of the principles and practical applications of Isothermal Titration Calorimetry (ITC), Differential Scanning Calorimetry (DSC), Circular Dichroism (CD) and Fluorescence Spectroscopy (FS). Through a combination of theoretical lectures and hands-on laboratory sessions, attendees will develop experimental competence, strengthen analytical thinking, and gain confidence in data interpretation. By the end of the course, participants will be equipped to apply these methods effectively in their own research projects.
The program will include lectures open to a broader audience, while practical sessions will be reserved for registered participants to ensure maximum hands-on experience. Participants will have the opportunity to measure their own samples upon discussion.
The practical part will be carried out on the instruments PEAQ-DSC Automated (Malvern), PEAQ-ITC Automated (Malvern), Chirascan V100 (Applied Photophysics), Fluorolog QM and Duetta (HORIBA).
Upon registration you will be asked to send CV and motivation letter - 16 participants for practicals will be selected according to specific criteria (carrier stage, institutional location, potential for know-how transfer, etc.) . The selected participants willbenefit from reimbursement of 200 €/person.
Deadline for registration to the whole workshop is 28th June 2026.
Speakers:
Tomáš Brom (CEITEC, CZ)
Markus Epe (Applied Photophysics, GE)
Josef Houser (CEITEC, CZ)
Raul Pacheco Gomez (Malvern, UK)
Grzegorz Piszczek (NIH - NHLBI, USA) - online
Peter W. Schuck (NIH - NIBIB, USA) - online
Martin Textor (Applied Photophysics, GE)
Huaying Zhao (NIH - NIBIB, USA) - online
Programme
Day 1:
12:30 – 13:00 Registration
13:00 – 13:10 Welcome
13:10 – 14:40 Lecture: Biomolecules and their interactions (Josef Houser)
14:40 – 15:00 Coffee break
15:00 – 16:00 Lecture: Sample quality assessment (Josef Houser)
16:00 – 17:00 Initial discussion of participants' scientific background and projects
17:00 – 18:00 Tour of CEITEC MU Core Facilities (optional)
Day 2:
09:00 – 10:30 Lecture: Biomolecular calorimetry – Introduction ITC (Raul Pacheco Gomez)
10:30 – 11:00 Coffee break
11:00 – 12:30 Lecture: Biomolecular calorimetry – Introduction DSC (Raul Pacheco Gomez)
12:30 – 13:30 Lunch break
13:30 – 15:00 Hands-on: Isothermal titration calorimetry/Differential scanning calorimetry
(Raul Pacheco Gomez/Jitka Holková)
15:00 – 15:30 Coffee break
15:30 – 17:00 Hands-on: Isothermal titration calorimetry/Differential scanning calorimetry
(Raul Pacheco Gomez/Jitka Holková)
17:00 – 18:00 Hands-on: Calorimetrical measurement of attendees’ samples (optional)
Day 3:
09:00 – 10:30 Lecture: Biomolecular calorimetry – Applications ITC (Raul Pacheco Gomez)
10:30 – 11:00 Coffee break
11:00 – 12:30 Lecture: Biomolecular calorimetry – Applications DSC (Raul Pacheco Gomez)
12:30 – 13:30 Lunch break
13:30 – 15:30 Hands-on: Calorimetry data evaluation (Raul Pacheco Gomez)
15:30 – 16:00 Coffee break
16:00 – 17:30 Hands-on: Complex data analysis using SEDPHAT
(Peter Schuck, Huaying Zhao, Grzegorz Piszczek - online presentation)
19:00 – 22:00 Social event (optional)
Day 4:
09:00 – 10:30 Lecture: Biomolecular fluorescence spectroscopy (TBA)
10:30 – 11:00 Case study: Novel approach to FRET measurement set-up and analysis (Tomáš Brom)
11:00 – 11:30 Coffee break + move to C04
11:30 – 13:00 Hands-on: Fluorescence spectroscopy Duetta /Fluorescence spectroscopy (TBA/Jitka Holkova)
13:00 – 14:00 Lunch break
14:00 – 15:30 Lecture: Circular dichroism spectroscopy (Markus Epe)
15:30 – 15:45 Coffee break
15:45 – 16:45 Lecture: Circular dichroism spectroscopy – applications (Martin Textor)
16:45 – 18:00 Hands-on: Circular dichroism (Martin Textor)
Day 5:
09:00 – 10:30 Hands-on: CD spectroscopy data evaluation (Martin Textor)
10:30 – 11:00 Coffee break
11:00 – 12:30 Hands-on: Fluorescence spectroscopy Duetta/Fluorescence spectroscopy (TBA/Jitka Holkova)
12:30 – 13:30 Lunch break
13:30 – 15:30 Hands-on: Spectroscopical measurement of attendees’ samples (optional)
15:30 – 16:00 Coffee break
16:00 – 17:00 Final discussion and course wrap-up
For more information contact bic@ceitec.cz
