Share
Junctions in Noncanonical Forms of Nucleic Acids
Doctoral study program
Life Sciences (Faculty of Science, Masaryk University)
Research area
Structural Biology
Supervisor
Annotation
The project is focused on detailed structural characterization of short oligonucleotides in noncanonical forms clipped together by proper sequential motifs. For this purpose, NMR experiments combined with MD simulations will be employed. The effect of modifications of selected nucleotides on the structural properties of designed models will be investigated to gain a deeper understanding of key interactions that contribute to the system folding and stability.
Recommended literature
- Aleš Novotný, Jan Novotný, Iva Kejnovská, Michaela Vorlíčková, Radovan Fiala, Radek Marek. Revealing structural peculiarities of homopurine GA repetition stuck by i-motif clip. Nucleic Acids Research, 2021, 49, 11425. doi:10.1093/nar/gkab915.
Funding
RG budget, planned Czech Science Foundation
Requirements on candidates
Structural chemistry or biology, advanced NMR spectroscopy, computational chemistry
Keywords
NMR spectroscopy, noncanonical DNA, base pairing, molecular modelling
Information on the supervisor
ResearcherID: D-6929-2012, currently 2 PhD students, 12 alumni