About event
Mass photometry is a label-free, single-molecule technique that measures the mass of biomolecules in solution with exceptional accuracy and sensitivity. By detecting the light scattered by individual molecules as they land on a glass surface, it enables the direct analysis of molecular mass, oligomeric states, and complex formation in real time. Mass photometry offers a rapid, non-destructive way to study proteins, nucleic acids, and biomolecular assemblies, making it a powerful tool for assessing sample purity, stoichiometry, and interactions under native conditions. Key applications include the analysis of AAV particles (distinguishing between empty and full capsids, assessing packaging efficiency, etc.) and the study of antibodies and their interaction.
Workshop is organized by Core Facility Biomolecular Interaction and Crystallography with collaboration of Refeyn and Specion.
Programme
September 4, 2025
Focus: Introduction to mass photometry and the instrumentation
- 09:30–09:40 Welcome and Opening Remarks
- 09:40–10:30 TwoMP: A UNIQUE TECHNIQUE FOR BIOMOLECULES, MFx OVERVIEW
Introduction to the principles and advantages of mass photometry. - 10:30–10:40 Coffee Break
- 10:40–11:00 CORE APPLICATIONS
Overview of biomolecular interactions and fundamentals of the MFx microfluidic system. - 11:00–11:30 CASE STUDY (Monika Kubíčková, Ph.D.)
- 11:30–12:30 Lunch Break
- 12:30–16:30 HANDS-ON SESSION: TwoMP
Live measurements of both reference and user-provided samples.
September 5, 2025
Focus: Advanced applications and fluidic workflows
- 09:30–10:30 DATA ANALYSIS
- 10:30–10:40 Coffee Break
- 10:40–11:30 DEEP DIVE INTO APPLICATIONS
Special use cases including Cryo-EM sample assessment and complex binding studies. - 11:35–12:30 Lunch Break
- 12:30–16:30 HANDS-ON SESSION: TwoMP AND MFx
User-driven experiments and discussions on specific applications.
