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About event
The CF Biomolecular Interactions and Crystallography, with support from the ARBRE association and National Centre for Biomolecular Research of MU, would like to invite you to a workshop focused on the characterization of biomolecular interactions. The workshop is intended for structural biologists, biochemists, and all others who wish to improve their knowledge of current methods for studying interactions between biomacromolecules and their ligands, including proteins, nucleic acids, and small molecules.
The workshop consists of theoretical lectures and practical sessions. The lectures (Mon-Tue) are opened to all interested participants upon registration, up to the capacity of the seminar room. The following practical part consisting of hands-on measurements using AUC, ITC, BLI, and MST, accompanied by data evaluation sessions will be restricted for capacity reasons. The ARBRE members will receive priority for participation in the practical sessions.
SPEAKERS:
Sébastien Brûlé (Institute Pasteur, fr)
Josef Houser (CEITEC, cz)
Jan Komárek (NCBR, cz)
Eva Paulenová (NCBR, cz)
Jitka Holková (CEITEC, cz)
Programme
Preliminary Program:
Monday 9th Feb
09:50 – 10:00 Welcome
10:00 – 11:00 Importance of sample preparation (Sébastien Brûlé)
11:00 – 12:30 Biomolecular interactions – introduction (Josef Houser)
lunch break
13:30 – 15:00 Spectroscopic methods (Sébastien Brûlé)
15:00 – 16:30 Isothermal titration calorimetry (ITC) (Jitka Holková)
Tuesday 10th Feb
09:00 – 10:30 Analytical ultracentrifugation (AUC) (Jan Komárek)
break
10:45 – 12:15 Surface-based methods (Josef Houser)
lunch break
13:15 – 14:15 Microscale thermophoresis (MST) (Eva Paulenová)
14:15 – 14:45 Interactions at the cell level (Josef Houser)
14:45 – 15:15 Databases for data deposition (presented by BIOCEV)
Wednesday 11th Feb
09:00 – 16:00 Practicals
Thursday 12th Feb
09:00 – 16:00 Practicals
Deadline for registration to practical part of course is 25th January 2026.
