Methods for Characterization of Biomolecular Interactions

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About event

The CF Biomolecular Interactions and Crystallography, with support from the ARBRE association and National Centre for Biomolecular Research of MU, would like to invite you to a workshop focused on the characterization of biomolecular interactions. The workshop is intended for structural biologists, biochemists, and all others who wish to improve their knowledge of current methods for studying interactions between biomacromolecules and their ligands, including proteins, nucleic acids, and small molecules. ​

The workshop consists of theoretical lectures and practical sessions. The lectures (Mon-Tue) are opened to all interested participants upon registration, up to the capacity of the seminar room. The following practical part consisting of hands-on measurements using AUC, ITC, BLI, and MST, accompanied by data evaluation sessions will be restricted for capacity reasons. The ARBRE members will receive priority for participation in the practical sessions.

POSTER

SPEAKERS:

Sébastien Brûlé (Institute Pasteur, fr)​

Josef Houser (CEITEC, cz)​

Jan Komárek (NCBR, cz)​

Eva Paulenová (NCBR, cz)​

Jitka Holková (CEITEC, cz)

Jitka Plucarová (BIOCEV, cz)

Jan Dohnálek (BIOCEV, cz)

Programme

Preliminary Program:

Monday 9th Feb

09:50 – 10:00  Welcome

10:00 – 11:00  Importance of sample preparation (Sébastien Brûlé)

11:00 – 12:30  Biomolecular interactions – introduction (Josef Houser)

            lunch break

13:30 – 15:00  Spectroscopic methods (Sébastien Brûlé)

15:00 – 16:30  Isothermal titration calorimetry (ITC) (Jitka Holková)

Tuesday 10th Feb

09:00 – 10:30  Analytical ultracentrifugation (AUC) (Jan Komárek)

             break

10:45 – 12:15  Surface-based methods (Josef Houser)

            lunch break

13:15 – 14:15  Microscale thermophoresis (MST) (Eva Paulenová)

14:15 – 14:45  Interactions at the cell level (Josef Houser)

14:45 – 15:45  Databases for data deposition (Jitka Plucarová, Jan Dohnálek, BIOCEV)

Wednesday 11th Feb

09:00 – 16:00  Practicals

Thursday 12th Feb

09:00 – 16:00  Practicals

Deadline for registration to the practical part of the course is 25^th January 2026.

We regret to inform you that, due to limited capacity, registration for the practical part is no longer available. At this time, registration is possible exclusively for the lecture sessions.