Multimodal Microscopy Workshop 2025

8:30 – 9:00 

Registration, coffee (E35, atrium)

9:00 – 9:15

Workshop opening – Microscopy and Its Importance in the Brno Region  (J. Neuman, NenoVision, Brno)

9:15 – 9:45 

Structure, mechanical properties, and chemical composition of biosamples by AFM, optical, and Raman microscopy  (J. Přibyl, CEITEC MU, Brno) 

9:45 – 10:00 

Company presentation – From Ångström to Zoology – Measuring Every Detail (J. Horák, Měřicí technika Morava, Czech Republic)

10:00 – 10:30

Coffee break (E35, atrium)

10:30 – 11:15

Invited lecture – Enhanced Atomic Force Microscopy by optical imaging techniques (D. Hadraba, Bioimaging Facility - CZBI, Institute of Physiology, Prague) 

11:15 – 11:45

Company presentation – Micro Meets Nano: Integration of Atomic Force Microscopy and Optical Techniques (A. Dulebo, JPK BioAFM, Bruker Nano GmbH, Berlin, Germany)

11:45 – 12:00

Controlled Release of Insoluble and Unstable Flavonoids from Magnetic Nanocarriers by External Magnetic Fields  (S. Šegota, IRB, Zagreb, Croatia)

12:00 – 13:30 

Lunch (E35, atrium)

13:30 – 14:00

Company presentation – Pioneering the Future of Microscopy: ZEISS Innovations in Light Microscopy (T. Cicerone, Carl Zeiss, Belgium)

Section 1

Visualization of a new type of samples
(15 min each) (E35, atrium)

14:00 – 15:00

• Imaging Extracellular Vesicles: From Light Microscopy to Cryo-EM (V. Pospíchalová, Faculty of Science, MU, Brno)
• New Immobilization Techniques (Š. Vrana, CEITEC MU, Brno) 
• Multimodal atomic force and optical microscopy reveal mechanical properties of multi-phase biomolecular condensates (J. Cibulka, Faculty of Science, MU, Brno) 
• Advanced Imaging of Lipid-Based Nanoparticles for Drug Delivery (M. Malásková, E. Maraková, CEITEC BUT, Brno)

15:00 – 15:30 

Leica Microsystems Product Portfolio (V. Koláčková, Specion, Praha)

15:30 – 16:00 

Coffee break (E35, atrium)

16:00 – 16:30 

Smart experiments, fast analysis: TI2-E and NIS Elements powered by AI (J. Karas, Nikon, Czech Republic)

Section 2

Fire talks (E35, atrium) (5 min each)

16:30 – 17:30 

• Bacterial biofilm with piezoelectric properties (M. Khýrová, Faculty of Chemistry, Brno University of Technology, Brno)
• Manufacturing of CAD/CAM crowns with and without a 3D printed model in terms of marginal fit (M. Al-Akkad, V. Prokupek, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic) 
• Vizualization of bacterial biofilm using AFM (B. Yöney, Faculty of Science, MU, Brno) 
• Measuring of SEM charging directly via AFM (J. Hruška, CEITEC MU, Brno)
• Fitting correlated data in AFM force distance curves (A. Ch. Campbell, Primary Nanometrology and Technical Length, Czech Metrology Institute, Brno) 
• High-Resolution Hyperspectral Microscopy for Efficient Analysis of Biological Systems (O. Vaculík, VUT, Brno)
• Molecular imprinting as a solution for environmental problems (E. Birgusová,  Mendel University in Brno)

8:30 – 9:00 

Getting together, coffee (E35, atrium)

9:00 – 9:45 

Company presentation – Live Demonstration of Optical Photothermal Infrared (O-PTIR) - Multimodal submicron IR, Simultaneous Raman and Fluorescence microscopy (Miriam Unger, Photothermal Spectroscopy Corp., Santa Barbara CA, USA)

Section 3

Emerging Approaches for Biomolecular Analysis Using AFM/Raman microscopy
(15 min each) (E35, atrium)

9:45 – 10:15

• Use of Localized Atomic Force Microscopy to improve imaging resolution (B. Papulová, CEITEC MU, Brno) 
• Antimicrobial peptides - Bilayers and AFM (R.  Obořilová, CEITEC MU, Brno)

10:15 – 10:45 

Coffee break (E35, atrium)

10:45 – 11:15 

Company presentation – Telight LiveCodim – Universal super-resolution platform (M. Krijt - Telight Brno)

11:15 – 11:30 

• Alkaptonuria, homogentisate mapping by Raman microscopy (Barbora Lišková, Faculty of Science, MU, Brno)  

11:30 – 12:00 

Company presentation – Correlative AFM-in-SEM analysis (V. Hegrová, NenoVision, Czech Republic)

12:00– 12:15

• 3D electromechanical cell biosensors for drug and disease screening (M. Pešl, Faculty of Medicine, MU, Brno)

12:15 – 13:30 

Lunch (E35, atrium)

Section 4

Afternoon practical session - Getting together
(E35, atrium)

13:30 – 17:30 

• Bruker FastScan – Bilayer rupture testing (E35, 2S014)
• Raman microscopy– Microplastic detection (E35, 2S014)
• JPK Nanowizard 4XP – Cellular biomechanics (E35, 2S012)
• JPK Nanowizard 3 – Cellular imaging (E35, 2S012)
• AFM in SEM (E35, 1S118)
• Commercial demos – Zeiss, Nikon, Specion (Leica) (E35, atrium)

19:00 – 22:30 

Workshop dinner (E35, atrium)

9:00 – 9:30 

Getting together, coffee (E35, CF labs)

9:30 – 12:30

Practical session (E35, CF labs)

• Bring your own sample
• Hands-on practice

11:30 – 12:00 

Coffee break (E35, atrium)

12:00 – 12:30 

Closing remarks (E35, atrium)