This is part of the Principal Investigator Seminar Series.
The detection of low analyte concentrations is of fundamental importance for early disease diagnosis as well as other applications. Single-molecule assays (or digital assays) enable new approaches to avoid optical background interference. I will highlight the advantages of UCNPs as luminescent labels for background-free imaging and single-molecule applications. Additionally, the talk will cover the use of femtoliter reaction vessels for analyzing the individual catalytic activity of single enzyme molecules. Such experiments have shown that there is a broad distribution of activities among individual enzyme molecules.
Hans H. Gorris studied biology at the University of Münster (Germany) and obtained his PhD from the University of Lübeck (Germany). During a postdoctoral stay with David Walt at Tufts University (USA), he switched to chemistry and then joined the University of Regensburg (Germany), where he served as the chair of a European COST Action on upconversion from 2014-2018 and held a prestigious Heisenberg-Fellowship from 2016 to 2020. In 2021, he was appointed Associate Professor at the Department of Biochemistry at Masaryk University in Brno (Czech Republic). His research is focused on background-free luminescent bioaffinity assays based on upconversion nanoparticles with single-molecule sensitivity as well as new methods for investigating enzyme kinetics at the single-molecule level.