Lecture Seminar Series

PI Seminar: Upconversion Microscopy for Background-free Imaging and Analysis of Enzyme Kinetics at the Single Molecule Level

About event

This is part of the Principal Investigator Seminar Series.



The detection of low analyte concentrations is of fundamental importance for early disease diagnosis as well as other applications. Single-molecule assays (or digital assays) enable new approaches to avoid optical background interference. I will highlight the advantages of UCNPs as luminescent labels for background-free imaging and single-molecule applications. Additionally, the talk will cover the use of femtoliter reaction vessels for analyzing the individual catalytic activity of single enzyme molecules. Such experiments have shown that there is a broad distribution of activities among individual enzyme molecules.

Hans H. Gorris studied biology at the University of Münster (Germany) and obtained his PhD from the University of Lübeck (Germany). During a postdoctoral stay with David Walt at Tufts University (USA), he switched to chemistry and then joined the University of Regensburg (Germany), where he served as the chair of a European COST Action on upconversion from 2014-2018 and held a prestigious Heisenberg-Fellowship from 2016 to 2020. In 2021, he was appointed Associate Professor at the Department of Biochemistry at Masaryk University in Brno (Czech Republic). His research is focused on background-free luminescent bioaffinity assays based on upconversion nanoparticles with single-molecule sensitivity as well as new methods for investigating enzyme kinetics at the single-molecule level.


16. 9. 2022, 13:00 - 14:00
Add to my calendar


Hans-Heiner Gorris, Masaryk University, Faculty of Science


University Campus Bohunice, Building B11, Room 205