Protein-DNA Interactions - Konstantinos Tripsianes
Protein-DNA Interactions - Konstantinos Tripsianes
Protein-DNA Interactions


We develop 4D-CHAINS, a software for fully automated protein backbone N-H and sidechain aliphatic C & H chemical shift assignment from 2 NMR spectra: a 4D TOCSY and a 4D NOESY. The program is free for non-commercial usage and can be downloaded from

The main advantage of the method is the use of 4D spectra that offer higher resolution and allow NMR studies of larger structures compared to traditional 3D NMR approaches. 4D-CHAINS was successful in assigning automatically a protein composed of 248 amino acids. More information about the algorithm and its performance can be found in the following publication: 

Evangelidis T, Nerli S, Nováček J, Brereton AE, Karplus PA, Dotas RR, Venditti V, Sgourakis NG, Tripsianes K, "Automated NMR resonance assignments and structure determination using a minimal set of 4D spectra".  Nat Commun. 2018 Jan 26;9(1):384.