About event
Hands-on workshop
Organized by Nanobio Core Facility
Lectures
Jan Pribyl (CEITEC MU):
AFM microscopy – introduction to the method, basic imaging modes and sample preparation.
Practical session with Bruker Dimension FastScan.
Petr Klapetek (CMI Brno):
Gwyddion software use for AFM data analysis.
Simon Klimovic (CEITEC MU):
Preparation of samples for AFM.
Hands-on session
- Immobilization of nanoparticles and proteins on mica surface.
- Bruker Dimension FastScan imaging of nanoparticels and proteins in the lab atmosphere and in liquids.
Register at pribyl@nanobio.cz
WORKSHOP SCHEDULE
Tuesday 30th July
- 8:45 Opening (Meet and Greet)
- 9:00-9:45 Lecture: “Introduction to AFM microscopy” Jan Pribyl (CEITEC MU) / Seminar room 211
- 9:50-10:35 Lecture: “Basic and advanced modes of AFM” Jan Pribyl (CEITEC MU) / Seminar room 211
- 10:40-11:00 Coffee break
- 11:00-11:50 Lecture: “Sample preparation for AFM microscopy” Jan Pribyl (CEITEC MU) / Seminar room 211
- 13:00-13:30 “Introduction to Nanobio Core Facility labs and equipment” Jan Pribyl and Simon Klimovic (CEITEC MU) / NanoBioCF Lab 2S014
- 13:30-15:30 Hands-on: “Sample preparation - immobilization of nanoparticles and proteins” Jan Pribyl and Simon Klimovic (CEITEC MU) / NanoBioCF Lab 2S014
- 15:30-16:00 Coffee break
- 16:00-18:00 Hands-on: “Basic imaging of samples in a tapping mode under laboratory atmosphere” Jan Pribyl and Simon Klimovic (CEITEC MU), Bruker Dimension FastScan AFM microscope / NanoBioCF Lab 2S014
Wednesday 31st July
- 8:45 Meet and Greet by coffee machine
- 9:00-11:45 Hands-on: “Advanced AFM modes for characterization of samples in a liquid environment -QNM, ScanAsyst, Force Volume” Jan Pribyl and Simon Klimovic (CEITEC MU), Bruker Dimension FastScan AFM microscope / NanoBioCF Lab 2S014
- 13:00-16:00 Lecture & Hands-on: “Gwyddion software for processing and presentation of AFM data” Petr Klapetek (CMI Brno), Bring your laptop – to follow the speaker and process your data. / Seminar room 211
- 16:00-16:20 Closing remarks