Telight Holographic Incoherenct-ligh-source Quantitative Phase Imaging (Q-Phase)

Profilová karta (PDF)

Description:

Q-Phase is a cutting-edge microscope system designed for high-precision, label-free quantitative phase imaging (QPI). Based on a patented technology of coherence-controlled holographic microscopy, Q-Phase uses an incoherent light source to deliver high-quality, artefact-free imaging—overcoming the common drawbacks of laser-based systems.

At the core of Q-Phase is a 660 nm LED light source, with the beam split into two paths: one passing through the sample, the other through a reference. Upon recombination, a digital hologram is generated, capturing both amplitude and phase information in real time. This approach enables non-invasive, high-contrast imaging with exceptional sensitivity.

In cell biology, QPI is particularly valuable: phase shifts directly correspond to cell dry mass, offering an accurate, quantitative metric for assessing cell health, growth, and behavior—without the need for labels or dyes.

Q-Phase is engineered for ease of use and experimental flexibility, featuring:
*Motorized hardware: Automated objective exchange, sample stage control, and focusing
*Automated acquisition: Time-lapse, multiposition, multichannel, and Z-stack capabilities
*Integrated fluorescence module: Seamlessly combine quantitative phase and fluorescence imaging for correlative, multimodal analysis
*Microscope incubator: Microscope-compatible incubator with adjustable temperature for live-cell experiments
*Advanced analysis software: Comprehensive tools for quantification, segmentation, and cell tracking

Key Features
*Quantitative Phase Imaging: Label-free, non-invasive imaging without halos, speckles, or parasitic interference
*Live-cell imaging: Low phototoxicity; ideal for long-term time-lapse and multi-field experiments
*Real-time quantification: Direct measurement of cell dry mass, without scanning
*Accurate segmentation & tracking: Clear backgrounds and precise cell boundary detection
*Multimodal capabilities: Overlay quantitative phase and fluorescence images for comprehensive insights
*High-contrast visualization: Capture fine cellular structures and compartments
*Imaging in scattering media: Effective in scattering and turbid media environments (callagen gells, 3D matrices)

Objective Lenses Nikon
*4× Plan Achromat, NA 0.1, WD 30 mm
*10× Plan Fluor, NA 0.3, WD 16 mm
*20× Plan Fluor, NA 0.5, WD 2.1 mm
*40× Plan Apo, NA 0.95, WD 0.17–0.25 mm

Fluorescence Filters
*DAPI / FITC / TRITC / Cy5
*GFP / mCherry

Applications Examples
*Long-term live-cell imaging, stem cells and cancer research
*Monitoring cellular growth throughout the cell cycle
*Studying morphology and dry mass changes after treatments
*Investigating different forms of cell death
*And many more applications in biology and beyond

Q-Phase is also suitable for non-biological and material science studies.

The system is available for scientific use, testing, and custom applications.

Developed in collaboration with Telight, Q-Phase incorporates a globally unique technology protected by patents in the EU, USA, and EA, with pending applications in other regions.

For the latest updates and detailed specifications, visit the Telight website.