Characterization of histone posttranslational modifications
The development of proteomic methodology with focus to plant proteomics
Content of research
Generally, our research interests focus on the development/optimization of all steps of proteomic workflow with aim to maximize information obtained by analysis of given sample. More specifically, we are mainly focused on:
MALDI-MS profiling – development of new sample preparation protocols improving the discriminatory power of the technique for distinguishing bacterial strains of closely related species; application of the technique for other types of samples (e.g. malting barley varieties, spider venoms, bacteriophages); development of techniques for rapid MALDI-MS profiling of N-glycans for differentiation of lung cancer types.
Histone posttranslational modifications - we develop robust bottom-up workflow for characterization of histone modifications dynamics, namely acetylations, methylations and phosphorylations with focus to plant samples.
Development of tools for proteomic data processing - we develop workflows for data processing using Knime platform in combination with software containers. SW tools are available for free testing at https://github.com/OmicsWorkflows.
We apply our expertise in cooperations covering wide range of research topics. As examples:
Pollen proteomics – cooperation with the team of David Honys from the Institute of Experimental Botany, Czech Academy of Sciences dealing with study of the role of cell–cell communication in male–female recognition during plant sexual reproduction.
Characterization of Dishevelled proteins – cooperation with the team of Vítězslav Bryja from Faculty of Science, Masaryk University dealing with characterization of posttranslational modification of these key players in Wnt signaling pathways.