LSM 980 (Zeiss) Laser Scanning Confocal Microscope (LSM 980)

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Guarantor: Aneta Křížová, Ph.D.
Instrument status: Operational Operational
Research group: Biophotonics Core Facility


Description:

At the heart of the LSM 980 is a highly efficient beam path combined with the Airyscan detector, which captures more light and spatial information than traditional confocal systems. This allows users to achieve enhanced resolution and signal-to-noise ratio, even under low-light conditions—ideal for live-cell and deep tissue imaging. When configured with Multiphoton excitation, the system enables gentle, long-term imaging of thick samples.


Specification:

Designed for scientific versatility, the LSM 980 supports a wide range of imaging modalities and experiment types, including super-resolution, spectral imaging, and fast, simultaneous multi-channel acquisition. 

The ZEISS LSM 980 integrates: 

  • Airyscan Super-Resolution Detector: Capture images with up to 1.7x improved resolution and enhanced signal-to-noise, even at low excitation power. 
  • Spectral Detection System: Flexible channel separation and unmixing for complex multicolor experiments. 
  • Fast Acquisition Modes: Including Multiplex modes for rapid, high-resolution time-lapse imaging. 
  • Live-Cell Imaging: Compatible with environmental control chambers for long-term experiments with minimal phototoxicity. 
  • Multiphoton Imaging: Deep imaging with infrared lasers for minimized photodamage and maximized tissue penetration. 
  • Advanced Image Analysis Tools: ZEISS ZEN software provides robust tools for 3D rendering, segmentation, colocalization, and tracking—streamlining data analysis workflows. 

Objective Lenses Zeiss 

  • 10× Plan-Apochromat, NA 0.45, WD 2.10 mm, Dry
  • 25× Plan-Apochromat, NA 0.80, WD 0.57 mm, Immersion correction – water is preferable 
  • 40× C-Apochromat, NA 1.20, WD 0.28 mm, Water, Coverslip thickness correction
  • 63× Plan-Apochromat, NA 1.40, WD 0.19 mm, Oil   

Excitation Lasers 

  • Laser scanning and Airyscan mode: 405 nm, 488 nm, 543 nm, 639 nm 
  • Multiphoton mode: CHAMELEON Ti: Sapphire Laser 680 nm – 1080 nm 

Detection Range 

  • 32-Channel Quasar detector 380 nm – 757 nm 

Camera

  • TELEDYNE Prime BSI Express

Head of Core Facility

Daniel Zicha
Daniel Zicha
Deputy Research Group Leader (R3)
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