Zeiss LSM 980 (LSM 980)

Profilová karta (PDF)

Description:

Biophotonics Core Facility is endowed with the newest fluorescent laser scanning confocal microscope LSM 980. It provides excellent imaging performance combined with low phototoxicity and high speed, high-quality imaging of optical sections and spectral multiplexing by simultaneous spectral detection of multiple labels, from 380 to 900 nm emission. A light-efficient beam path with up to 34 simultaneous channels and full spectral flexibility into the near-infrared (NIR) range gives you the perfect basis for multicolour experiments with living samples.

Life-cell imaging needs special support - please in case you want to do imaging, contact one member of staff (D. Zicha / T. Chmelíková).

LSM 980 in combination with laser point illumination, linear scanning and detectors that can capture the signal in photon counting mode offers this imaging:
- Raster Image Correlation Spectroscopy (RICS) – generate a display map of molecule concentration and diffusion coefficients of a complete image frame of a cell, or other structures.
- Fluorescence Correlation Spectroscopy (FCS) – allows a non-invasive insight into molecular concentration and diffusion processes, leading to a deeper understanding of cell functions. To measure on a single molecule basis, you can use single photon laser lines and use the full emission range up to 900 nm.
- Fluorescent Cross-Correlation Spectroscopy (FCCS) – allows you to observe molecular interaction between two or more differentially labelled molecules by utilizing the 32 channels of the Quasar and performing FCCS with up to 7 individual channels.
- Förster Resonance Energy Transfer (FRET) – is another method for investigating molecular distances or interactions, using sensitized emission or acceptor photobleaching approaches.
- Fluorescence Recovery after Photobleaching (FRAP) – utilizes any laser lines to perform flexible photobleaching experiments. The same principle adheres to photomanipulation experiments in general, for example to investigate intracellular movement. Or follow cellular movement within whole organisms by photoconversion of fluorescent protein labels.
- Multilabel experiments: multichannel imaging of specimens labelled with fluorophores (e.g. CFP, GFP, YFP, Alexa Fluore 488/546/647 and Cy5) and some UV fluorophores (e.g. DAPI) in combination with transmitted light (including DIC) imaging
- Environmental chamber: temperature, humidity and CO2 control
- Z-series, Time-lapse, Multi-filed

Mechanical stage
- 130x85 R/L Set with a short coaxial drive for LSM incl. Mounting frame K (160mm x 110mm), suitable for Z-Piezo