Zeiss Laser Scanning Confocal Microscope (LSM 980)

Profilová karta (PDF)

Description:

At the heart of the LSM 980 is a highly efficient beam path combined with the Airyscan detector, which captures more light and spatial information than traditional confocal systems. This allows users to achieve enhanced resolution and signal-to-noise ratio, even under low-light conditions—ideal for live-cell and deep tissue imaging. When configured with Multiphoton excitation, the system enables gentle, long-term imaging of thick samples.

Designed for scientific versatility, the LSM 980 supports a wide range of imaging modalities and experiment types, including super-resolution, spectral imaging, and fast, simultaneous multi-channel acquisition.

The ZEISS LSM 980 integrates:
*Airyscan Super-Resolution Detector: Capture images with up to 1.7x improved resolution and enhanced signal-to-noise, even at low excitation power.
*Spectral Detection System: Flexible channel separation and unmixing for complex multicolor experiments.
*Fast Acquisition Modes: Including Multiplex modes for rapid, high-resolution time-lapse imaging.
*Live-Cell Imaging: Compatible with environmental control chambers for long-term experiments with minimal phototoxicity.
*Multiphoton Imaging: Deep imaging with infrared lasers for minimized photodamage and maximized tissue penetration.
*Advanced Image Analysis Tools: ZEISS ZEN software provides robust tools for 3D rendering, segmentation, colocalization, and tracking—streamlining data analysis workflows.

Objective Lenses Zeiss
*10× Plan-Apochromat, NA 0.45, WD 2.10 mm, Dry
*25× Plan-Apochromat, NA 0.80, WD 0.57 mm, Immersion correction – water is preferable
*40× C-Apochromat, NA 1.20, WD 0.28 mm, Water, Coverslip thickness correction
*63× Plan-Apochromat, NA 1.40, WD 0.19 mm, Oil

Excitation Lasers
*Laser scanning and Airyscan mode: 405 nm, 488 nm, 543 nm, 639 nm
*Multiphoton mode: CHAMELEON Ti: Sapphire Laser 700 nm – 1000 nm

Detection Range
*32-Channel Quasar detector 380 nm – 757 nm