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Abstracts
Invited lectures
Digital Holographic Microscopy for Cytometry and Histology
Dr. Björn Kemper, Head of Optical Technologies, Biomedical Technology Center of the Medical Faculty, University of Muenster, Germany.
- In an overview, concepts and systems for digital holographic microscopy (DHM)-based quantitative phase imaging (QPI) with focus on automated multimodal imaging for enhanced throughput are presented. The capabilities of QPI with DHM are illustrated by selected biomedical applications. These include utilization of DHM as tool for determining cell motility and growth dynamics during in vitro drug and toxicity testing based on physical and morphology related parameters down to the single cell level. In addition, the potential in histology is demonstrated by the quantification of inflammation and nanomaterial-induced pathological alterations in ex vivo colon and lung tissue sections. Results from holographic image flow cytometry and perioperative monitoring of living primary human blood cells after cardiac surgery prospect DHM as a cytometric tool for temporal disease course characterization.
Metrological characterization and validation of QPI systems in life sciences
Prof. Malgorzata Kujawinska, Institute of Micromechanics and Photonics, Warsaw University of Technology, Poland.
- 2D and 3D Quantitative Phase Imaging (QPI) is particularly useful as advanced imaging and measurement tool in biomedical research, but its performance is sample-dependent and cannot be reliably characterized with conventional approach to metrology. In order to characterize and benchmark wide range of QPI systems, it is crucial to use phantoms that represent key details of the sample in realistic working conditions, and evaluate the systems in an end-to-end fashion. This presentation summarizes the metrological needs and protocols for the QPI systems, as well as presents 3D-printed microphantoms ranging from simple bars, steps, cells and cell cultures, up to volumetric and scattering phantoms mimicking tissues or organoids. Such phantoms are characterized by traceable and calibrated instruments and therefore they seem to be invaluable tool to certify QPI instruments and enable reliable measurement outcomes.
Real Time Acquisition and Display of En-face Optical Coherence Tomography
Prof. Adrian Podoleanu, Applied Optics Group, University of Kent, Canterbury, United Kingdom.
- Optical Coherence Tomography (OCT) has been initially developed as a non invasive high resolution optical imaging modality for ophthalmology, followed by rolling the technology to other medical fields as well as to non destructive testing. I will present the bottleneck in digital signal processing raised by the combination of an interferometer with a multi MHz tuning rate laser as well as solutions to display en-face OCT slices in real time. We proposed an alternative OCT method that does not rely on a Fourier Transform and changes the main result of signal processing, from information along the axial coordinate to that along the transversal coordinate. This method, of Leader Follower (LF) [1] and its several facets, Complex Leader Follower (CLF) [2] and Downconversion Leader Follower (DLF) [3] will be presented. DLF can serve delivery of en-face OCT images in principle at any high sweeping rates, of MHz or tens of MHz.
- I will also show how Master students from Brno University of Technology have contributed to the progress of OCT through numerous regular exchange stages in Kent over the last 15 years.
Computational lensless holographic imaging from deep-UV to near-IR on a standard sensor.
Prof. Maciej Trusiak, Institute of Micromechanics and Photonics, Faculty of Mechatronics, Warsaw University of Technology, Poland.
- Lensless holographic microscopy offers a powerful route to high-content two- and three-dimensional imaging over large fields of view, but its broader use is often constrained by limited chemical specificity and by multiple scattering in optically dense samples. In this lecture, I will present a unified lensless imaging framework implemented on a standard CMOS camera and operating across an exceptionally wide spectral range, from deep ultraviolet (~240 nm) to the silicon cut-off near 1100 nm. This platform enables UV absorption imaging with intrinsic chemical contrast, near-infrared complex-field imaging with reduced scattering in turbid specimens, and multiple-scattering-aware phase tomography at gigavoxel scale. Together, these advances show how a simple detector platform can support computational microscopy and tomography across complementary spectral regimes, opening new possibilities for high-throughput and information-rich imaging of complex samples.
From Quantitative Phase Imaging to Neuropsychiatric Disorders: Twenty Years of Digital Holographic Microscopy
Prof. Pierre Marquet, Ing. Phys. (EPFL), MD, PhD, FMH, Centre for Psychiatric Neuroscience (CNP), Lausanne University Hospital (CHUV), Switzerland; CERVO Brain Research Centre, Université Laval, Québec, Canada.
- Over the past two decades, digital holographic microscopy (DHM) has evolved from a label-free optical imaging technique into a quantitative tool for probing cellular structure and dynamics. This lecture will present key instrumental advances, including polychromatic DHM, enabling high-precision phase measurements, together with strategies to translate quantitative phase signals into biophysical cellular parameters and processes. Combined with iPSC-derived human neurons and artificial intelligence, this approach enables the identification of disease-specific functional signatures of neuropsychiatric disorders. It reveals promising biomarkers for bipolar disorder and schizophrenia, with potential impact on diagnosis, treatment, and mechanistic understanding.
