Axio Observer Life Cell Imaging (Zeiss) (Axio Observer Life Cell Imaging)

Profilová karta (PDF)

Description:

The ZEISS Axio Observer is a versatile inverted microscope platform designed for advanced live-cell imaging and long-term time-lapse experiments. Engineered with stability, precision, and flexibility in mind, it enables researchers to capture high-quality images of dynamic biological processes while maintaining optimal physiological conditions for living samples.

Specification:

By integrating cutting-edge imaging modules, environmental control systems, and advanced software, the Axio Observer provides an adaptable foundation for diverse applications—from fluorescence and phase contrast imaging. 

The ZEISS Axio Observer integrates: 

• Stable Inverted Platform: Optimized for live-cell imaging with minimized drift, ensuring consistent results during long-term experiments. 
• Environmental Control: Supports incubation chambers to maintain temperature, CO₂, humidity, and O₂ levels for extended live-cell studies. 
• Advanced Contrast Techniques: Compatible with widefield fluorescence, phase contrast,  and transmitted light. 
• High-Performance Automation: Motorized focus, stage, and filter wheels for precise, reproducible imaging workflows. 
• ZEN Imaging Software: Intuitive acquisition and analysis tools for time-lapse imaging, multi-channel experiments, colocalization, and 3D rendering. 

Objective Lenses Zeiss: 

• 2.5× EC Plan-Neofluar, NA 0.085, WD 8.8 mm, Dry 
• 10× EC Plan-Neofluar, NA 0.3, WD 5.2 mm, Ph1, Dry 
• 20× LD Plan-Neofluar, NA 0.4, WD 7.9 mm, Ph2, Dry 
• 40× LD Plan-Neofluar, NA 0.6, WD 2.9 mm, Ph2, Dry 
• 63× LD Plan-Neofluar, NA 0.75, WD 1.7 mm, Ph2, Dry 

Light Sources: 

• LED Viluma: UV 385nm, Violet 423nm, Blue 469nm, Cyan 508nm, Green 555nm, Red 630nm 
• Transmitted-light illuminator 

Filter sets: 

• 49 DAPI: Ex: 335-383nm, Em: 420-470nm 
• 38 eGFP: Ex: 450-490nm, Em: 500-550nm 
• 20 Rhodamine: Ex: 540-552nm, Em: 575-640nm 
• 50 Cy5: Ex:625-655nm, Em: 665-715nm 

Camera: 

• Axiocam 820 mono 

Applications Examples: 

• Multi-channel fluorescence acquisition 
• Long-term live-cell imaging  
• Cell tracking, segmentation, and analysis