CF: Core Facility Genomics

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Head of Core Facility

Boris Tichý, Ph.D. Boris Tichý, Ph.D. Head of Core Facility
Phone: +420 54949 8317, +420 733 141 527
Email:
Office:

Main Activity

Core Facility Genomics (CFG) offers genomic analysis service and access to up-to-date genomic instrumentation. Main technologies available at the facility are massively parallel sequencing (Illumina), single molecule nanopore sequencing, microarrays and high-throughput & digital PCR. Through cooperation with CEITEC Center of Molecular Medicine it is possible to access other instrumentation – cell sorter, flow cytometers or Laser Capture Microdissection.

Unique Features

Combination of high-end equipment and expertise for the complete experimental workflow from advanced sample preparation to complex genome analysis. Precise sample preparation techniques (cell sorting, microdissection) followed by combination of various complementary approaches in the analysis of the genome (massive parallel sequencing, microarrays, quantitative PCR) will make it possible to perform even very complex experimental designs including single cell genomics or diseased vs. healthy cells genome and transcriptome analyses.

Services and Methodologies Provided

• Next-generation sequencing (NGS)

• NGS library preparation

• NGS library quality control

• Single molecule nanopore sequencing

• Microarrays

• Flow cytometry

More detailed information ablout our services.

Key Equipment

• Massive parallel sequencers Illumina NextSeq and MiSeq

• Microarray system Agilent SureScan

• qPCR & digitalPCR

• Roche LC480

• ThermoScientific QuantStudio 12k

• Bio-Rad QX200

• Flow cytometry

• Beckton Dickinson FACS Aria Fusion

• Beckton Dickinson FACS Verse

• Single-cell

• Fluidigm C1

 

How to acknowledge this Facility

If you've used our Facility, please recognise and acknowledge it in your work. In a scientific publication that includes data generated within the Facility, please use the following statement:

We acknowledge the CF Genomics CEITEC MU supported by the NCMG research infrastructure (LM2015091 funded by MEYS CR) for their support with obtaining scientific data presented in this paper.

 

The Core Facility is part of National Center for Medical Genomics (NCMG) research infrastructure funded by the Ministry of Education, Youth and Sports of the Czech Republic.